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Merck
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安全性情報

05-368MG

Sigma-Aldrich

Anti-phospho-Ser/Thr-Pro MPM-2 Antibody

clone MPM-2, Upstate®, from mouse

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About This Item

UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41
クローン:
MPM-2, monoclonal
application:
ELISA
IHC
IP
WB
化学種の反応性:
vertebrates
テクニック:
ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
citations:
11

由来生物

mouse

品質水準

抗体製品の状態

purified immunoglobulin

抗体製品タイプ

primary antibodies

クローン

MPM-2, monoclonal

化学種の反応性

vertebrates

メーカー/製品名

Upstate®

テクニック

ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

アイソタイプ

IgG1

輸送温度

wet ice

遺伝子情報

human ... FOXM1(2305)

詳細

This antibody recognizes a variety of proteins that are phosphorylated during mitosis.
Upon entry into M (Mitosis) phase, many proteins are phosphorylated either directly or indirectly by M-phase-promoting factor (MPF). The MPM2 monoclonal antibody binds to a phospho amino acid-containing epitope (peptides containing LTPLK and FTPLQ domains) present on more than 50 proteins of M-phase eukaryotic cells.

特異性

Recognizes phosphorylated serines or threonines when followed by a proline.

免疫原

Mitotic human HeLa cell cytosolic lysate

アプリケーション

Research Category
エピジェネティクス及び核内機能分子
Research Sub Category
細胞周期、DNA複製及び修復
Detect phospho-Ser/Thr-Pro MPM-2 also known as Mitotic protein #2 with Anti-phospho-Ser/Thr-Pro MPM-2 Antibody (Mouse Monoclonal Antibody), that has been demonstrated to work in ELISA, IHC, IP & WB.

品質

routinely evaluated in immunoblot on colcemid treated human HeLa carcinoma cells

ターゲットの説明

varies depending on the protein detected

物理的形状

Protein G Purified
Format: Purified
Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in mouse IgG in 1.0 mL PBS containing no preservatives.

保管および安定性

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

アナリシスノート

Control
Colcemid-treated HeLa (lane 2) cell lysates, A431 mitotic cells fixed with 3.7% paraformaldehyde

法的情報

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

12 - Non Combustible Liquids

WGK

WGK 2

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

05-368MG:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Rong Sang et al.
Cell & bioscience, 12(1), 78-78 (2022-06-02)
Mental retardation is a complex neurodevelopmental disorder. NPAT, a component of the histone locus body (HLB), has been implicated as a candidate gene for mental retardation, with a mechanism yet to be elucidated. We identified that mxc, the Drosophila ortholog
Mitosis-specific monoclonal antibody MPM-2 inhibits Xenopus oocyte maturation and depletes maturation-promoting activity.
Kuang, J, et al.
Proceedings of the National Academy of Sciences of the USA, 86, 4982-4986 (1989)
Phosphoproteins are components of mitotic microtubule organizing centers.
Vandre, D D, et al.
Proceedings of the National Academy of Sciences of the USA, 81, 4439-4443 (1984)
Makoto T Hayashi et al.
Nature, 522(7557), 492-496 (2015-06-26)
Tumour formation is blocked by two barriers: replicative senescence and crisis. Senescence is triggered by short telomeres and is bypassed by disruption of tumour-suppressive pathways. After senescence bypass, cells undergo crisis, during which almost all of the cells in the
Sequence-specific and phosphorylation-dependent proline isomerization: a potential mitotic regulatory mechanism.
Yaffe, M B, et al.
Science (New York, N.Y.), 278, 1957-1960 (1997)

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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