16-213

Anti-Myc Tag Antibody, clone 4A6, HRP conjugate

clone 4A6, Upstate^®, from mouse

• 別名: Transcription factor p64, avian myelocytomatosis viral oncogene homolog, myc proto-oncogene protein, v-myc avian myelocytomatosis viral oncogene homolog, v-myc myelocytomatosis viral oncogene homolog (avian)
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.56
• 結合体: peroxidase conjugate
• application: WB
• クローン: 4A6, monoclonal
• 化学種の反応性: human
• citations: 14
• テクニック: western blot: suitable

特性

• 由来生物: mouse
• 品質水準: 100
• 結合体: peroxidase conjugate
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 4A6, monoclonal
• 化学種の反応性: human
• メーカー／製品名: Upstate^®
• テクニック: western blot: suitable
• NCBIアクセッション番号: NP_002458
• UniProtアクセッション番号: P01106
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... MYC(4609)

詳細

詳細

Immunogen: peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc

Specificity: Recognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts. Also recognizes human Myc.

Species Cross-reactivity: Human, others not tested

Ig Type: IgG

Host: Mouse

Molecular Weight: Varies depending upon the protein which is tagged

Myc-Tag is a short synthetic peptide sequence derived from C-terminal region of human c-myc that is used as an epitope tag. The Myc tag can be cloned either upstream or downstream of the target gene, resulting in a fusion protein that allows efficient purification, detection, and localization of target proteins. The relatively small size of this tag vs. other commonly used tags such as GST (30 kDa) makes it less likely to interfere with target protein structure or function. The myc epitope tag is widely used to detect expression of recombinant proteins in bacterial, yeast, insect, and mammalian systems.

特異性

Other species cross-reactivity not tested.

Recognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts. Also recognizes human Myc.

免疫原

Epitope: C-terminal

KLH-conjugated, synthetic peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc. Clone 4A6.

アプリケーション

Research Category
エピトープタグ抗体及び凡用抗体

Research Sub Category
エピトープタグ

Anti-Myc Tag Antibody, clone 4A6, HRP conjugate detects level of Myc Tag & has been published & validated for use in WB.

品質

Evaluated by Western Blot on Myc-PP2A A subunit transfected NIH/3T3 lysates.

Western Blot Analysis:
1:500 dilution of this antibody detected myc-PP2A on 10 µg of Myc-PP2A A subunit transfected NIH/3T3 lysates.

ターゲットの説明

Varies depending upon the protein which is tagged.

物理的形状

Protein G Purified

Purified mouse monoclonal IgG1 conjugated to horseradish peroxidase (HRP) in buffer containing: 50% storage buffer (PBS, 0.05% Kathon^®, 0.2 mg/mL BSA) and 50% glycerol.

保管および安定性

Stable for 1 year at -20ºC from date of receipt.

アナリシスノート

Control
Positive control for human Myc: Widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

Kathon is a registered trademark of Rohm and Haas Co.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• ピクトグラム: GHS07,GHS09
• シグナルワード: Warning
• 危険有害性情報: H317,H411
• 注意書き: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• 危険有害性の分類: Aquatic Chronic 2 - Skin Sens. 1
• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): 390.2 °F - closed cup - Information refers to the main ingredient.
• 引火点(℃): 199 °C - closed cup - Information refers to the main ingredient.