322133

DSP, Water-Soluble

• 別名: 3,3-Dithio- bis-(sulfosuccinimidyl)propionate, DTSSP, Sulfo-DSP, 3,3-Dithio-bis-(sulfosuccinimidyl)propionate, DTSSP, Sulfo-DSP
• UNSPSCコード: 41116133
• NACRES: NA.24

特性

• アッセイ: ≥94% (HPLC)
• 品質水準: 100
• 形状: solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; desiccated
• 色: off-white to light tan
• 溶解性: water: 6 mg/mL
• 輸送温度: ambient
• 保管温度: 2-8°C

詳細

詳細

Bifunctional cross-linker. The disulfide bond can be cleaved with 1% NaBH₄, 50 mM DTT, or 100 mM β-mercaptoethanol. Has been used to immobilize proteins on supports containing amino groups.

Bifunctional cross-linker. The disulfide bond can be cleaved with 1% NaBH₄, 50 mM DTT, or 100 mM β-mercaptoethanol. Has been used to immobilize proteins on supports containing amino groups. Chemical crosslinkers are used to covalently modify proteins for studying ligand receptor interactions, conformational changes in tertiary structure, or for protein labeling. Crosslinkers are divided into homobifunctional crosslinkers, containing two identical reactive groups, or heterobifunctional crosslinkers, containing two different reactive groups. Water-soluble cross-linking reagents help to avoid the use of organic solvents such as dimethylformamide (DMF) and dimethylsulfoxide (DMSO) when preparing bioconjugates.

警告

Toxicity: Irritant (B)

再構成

Following each use, purge the original vial with inert gas and refrigerate (4°C). Unstable in aqueous solution; reconstitute just prior to use. Alternatively, the solid DSP can be added directly to the sample to minimize hydrolysis. Hydrolysis is more pronounced in dilute protein solutions and can, therefore, be minimized by using more concentrated protein solutions (>5 mg/ml). For storage of stock solutions, reconstitute in anhydrous DMSO.

その他情報

Howard, A.D., et al. 1985. J. Biol. Chem.260, 10833.
Lee, W.T., and Conrad, D.H. 1985. J. Immunol.134, 518.
D′Souza, S.E., et al. 1988. J. Biol. Chem.263, 3943.
Yamada, H., et al. 1981. Biochem.20, 4836.
Annunziato, M.E., et al. 1993. Bioconjugate Chem.4, 212.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable