324717

Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli

Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide.

• 別名: Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli, Endo-β-N-acetylglucosaminidase H, Endo H
• CAS番号: 52769-51-4
• Enzyme Commission number: 3.2.1.96 (BRENDA, IUBMB)
• MDL番号: MFCD03791122
• UNSPSCコード: 12352202
• NACRES: NA.54

特性

• リコンビナント: expressed in E. coli
• 品質水準: 100
• 結合体: (N-linked)
• フォーム: liquid
• 比活性: ≥3 units/mL; ≥30 units/mg protein
• メーカー／製品名: Calbiochem^®
• 保管条件: do not freeze
• その他の活性: N-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase, neuraminidases, proteases, none detected
• 輸送温度: wet ice
• 保管温度: 2-8°C

詳細

詳細

Note: 1 mU = 1 milliunit.

Recombinant, streptomyces plicatus endoglycosidase H expressed in E. coli. Catalyzes the hydrolysis of asparagine-linked oligomannose and hybrid, but not complex oligosaccharides from glycoprotein. Endo H cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide.

警告

Toxicity: Standard Handling (A)

単位の定義

One unit is defined as the amount of enzyme that will catalyze the release of N-linked oligosaccharides from 1.0 µmol denatured ribonuclease B per min at 37°C, pH 5.5. Cleavage is monitored by SDS-PAGE.

物理的形状

In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.

その他情報

Rao, V., et al. 1995. Structure 3, 449.
Trimble, R.B., et al. 1987. Methods Enzymol. 138, 763.
Trumbly, R.J., et al. 1985. J. Biol. Chem.260, 5683.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable