324831

EGF human

≥95% (SDS-PAGE), recombinant, expressed in E. coli, solid, suitable for cell culture

• 別名: EGF, Human, Recombinant, E. coli, Epidermal Growth Factor, Human, Recombinant, E. coli
• CAS番号: 62229-50-9
• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• product name: EGF, Human, Recombinant, E. coli,
• リコンビナント: expressed in E. coli
• 品質水準: 100
• アッセイ: ≥95% (SDS-PAGE)
• 形状: solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• 不純物: <1.0 EU/μg Endotoxin (EU/μg EGF)
• 輸送温度: ambient
• 保管温度: −20°C

詳細

詳細

Recombinant, human epidermal growth factor (54 amino acids) expressed in E. coli. Identical to native human EGF except for an additional N-terminal methionine. Human EGF is 70% homologous to mouse EGF and has been shown to have similar biological activity. Mitogenic for a wide range of ectoderm- and endoderm-derived cells from tissue such as breast, cornea, epidermis, dermis, liver, pancreas, nerve, amnion, and adrenal medulla. Binding of EGF to its 170 kDa transmembrane receptor results in dimerization of the receptor, activation of the intrinsic receptor tyrosine kinase, and initiation of the complex signal transduction cascade that leads to cell proliferation and/or differentiation. Acts as a survival factor in preventing apoptosis.

Recombinant, human epidermal growth factor (54 amino acids) expressed in E. coli. Identical to human EGF except for an additional N-terminal methionine. Human EGF is 70% homologous to mouse EGF and has been shown to have similar biological activity. Mitogenic for a wide range of ectoderm- and endoderm-derived cells from tissue such as breast, cornea, epidermis, dermis, liver, pancreas, nerve, amnion, and adrenal medulla. Acts as a survival factor in preventing apoptosis.

生物化学的／生理学的作用

ED₅₀ = 100-400 pg/ml as measured by its ability to stimulate the ³H-thymidine incorporation in an EGF-responsive mouse fibroblast cell line, Balb/3T3.

警告

Toxicity: Standard Handling (A)

再構成

Following reconstitution, aliquot and freeze (-20°C or -70°C) for long term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C or -70°C.

Reconstitute to a concentration of ≥10 µg/ml in 10 mM acetic acid containing ≥0.1% HSA or BSA.

その他情報

Merlo, G.R., et al. 1995. J. Cell Biol. 128, 1185.
Yoshida, T., et al. 1993. Brain Res. Dev.76, 147.
Rubin, J.S., et al. 1991. Proc. Natl. Acad. Sci. USA88, 415.
Carpenter, G., and Wahl, M.I. 1990. In The Epidermal Growth Factor Family in Peptide Growth Factors and Their Receptors (Sporn, M.B., and Roberts, A.B., eds.) Springer-Verlag, New York, p 69.
Downward, J., et al. 1984. Nature311, 483.
Gray, A., et al. 1983. Nature303, 722.
Scott, J., et al. 1983. Science221, 236.
Cohen, S. 1960. Proc. Natl. Acad. Sci. USA46, 302.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 2