524627

Phosphatase Inhibitor Cocktail III

liquid, for the inhibition of serine/threonine and protein tyrosine phosphatases

• 別名: Phosphatase Inhibitor Mix (Set III), Phosphatase inhibitor
• UNSPSCコード: 12352200
• NACRES: NA.77

特性

• product name: Phosphatase Inhibitor Cocktail Set III, Phosphatase Inhibitor Cocktail Set III is a ready to use cocktail of four phosphatase inhibitors for broad-spectrum inhibition of both serine/threonine and protein tyrosine phosphatases.
• 形状: liquid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• 輸送温度: wet ice
• 保管温度: −20°C

詳細

詳細

Phosphorylation and dephosphorylation regulate signal transduction processes involved in cellular activities such as cell division, proliferation, and apoptosis. Protein kinases add a phosphate group to specific amino acid residues (serine, threonine, or tyrosine) on target proteins, changing their conformation and interaction with ligands. Protein phosphatases remove the phosphate group, restoring the protein to its dephosphorylated state. When extracting phosphorylated proteins from cell and tissue lysates, it is crucial to maintain their phosphorylation status intact. Phosphatase Inhibitor Cocktail Set III is a cocktail of four phosphatase inhibitors for broad-spectrum inhibition of both serine/threonine and protein tyrosine phosphatases. Available as a 1 ml vial or as a set of five 1 ml vials.

アプリケーション

Phosphatase Inhibitor Cocktail Set III has been used:

• as a supplement in lysis buffer to prepare protein lysates of whole testes for western blotting
• to fortify ice-cold lysis radio-immunoprecipitation assay (RIPA) buffer to homogenize larval hindguts for western blot analysis
• as a component in cell lysis buffer to extract total proteins for western blotting

生物化学的／生理学的作用

Cell permeable: no

Primary Target
Serine/threonine and protein tyrosine phosphatases

Product does not compete with ATP.

Reversible: no

特徴および利点

• Ready-to-use formulations for convenient use
• High-quality products for consistent and reproducible performance,
• Flexible options with a range of cocktails targeting different categories of phosphatases

構成

Each vial contains 1 ml of aqueous solution with the following components:

• 50 mM Sodium Fluoride
• 10 mM ß-Glycerophosphate (Cat. No. 35675)
• 10 mM Sodium Pyrophosphate Decahydrate
• 1 mM Sodium Orthovanadate

警告

Toxicity: Toxic (F)

物理的形状

In H₂O.

再構成

For long-term storage, aliquot and freeze (-20°C). Dilute 1:5 just prior to use.

その他情報

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable