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由来生物
human plasma
品質水準
形状
liquid
メーカー／製品名
Novagen®
保管条件
OK to freeze
avoid repeated freeze/thaw cycles
テクニック
protein extraction: suitable
適合性
suitable for additive or modifier in the separation of proteins or peptides
アプリケーション
life science and biopharma
輸送温度
wet ice
保管温度
−20°C
詳細
Restriction Grade Thrombin is a highly purified preparation qualified to specifically cleave target proteins produced with appropriate vectors. This preparation is functionally tested for activity with fusion proteins and is free of detectable contaminating proteases. Prepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV. Thrombin is supplied as a solution in 50% glycerol and includes 10X Thrombin Cleavage Buffer and a Cleavage Control Protein.
構成
•50 UThrombin
•1 ml10X Thrombin Cleavage Buffer
•2 ml1X Thrombin Dilution/Storage Buffer
•10 µgCleavage Control Protein
•1 ml10X Thrombin Cleavage Buffer
•2 ml1X Thrombin Dilution/Storage Buffer
•10 µgCleavage Control Protein
警告
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
単位の定義
One unit is defined as the amount of enzyme needed to cleave 1 mg of fusion protein in 16 hours at 20°C in a 200 µl reaction containing 20 mM Tris-HCl pH 8.4, 150 mM NaCl, 2.5 mM CaCl₂, 50 µg fusion protein.
調製ノート
Prepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
法的情報
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
保管分類コード
10 - Combustible liquids
WGK
WGK 1
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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Jooyoung Park et al.
eLife, 8 (2019-12-20)
The computational design of a symmetric protein homo-oligomer that binds a symmetry-matched small molecule larger than a metal ion has not yet been achieved. We used de novo protein design to create a homo-trimeric protein that binds the C3 symmetric
Christian M Smolko et al.
Scientific reports, 9(1), 19409-19409 (2019-12-21)
Protein kinases are enzymes whose abundance, protein-protein interactions, and posttranslational modifications together determine net signaling activity in cells. Large-scale data on cellular kinase activity are limited, because existing assays are cumbersome, poorly sensitive, low throughput, and restricted to measuring one
資料
Find highly-specific cleavage enzymes for isolating proteins during fusion protein purification including enterokinase, factor Xa, HRV 3C protease, and thrombin.
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