8.51086

Oxyma Pure

≥99.0% (HPLC), for peptide synthesis, Novabiochem^®

• 別名: Oxyma Pure, Ethyl cyano(hydroxyimino)acetate
• 実験式（ヒル表記法）: C₅H₆N₂O₃
• CAS番号: 3849-21-6
• 分子量: 142.11
• MDL番号: MFCD00002112
• UNSPSCコード: 12352126
• EC Index Number: 223-351-3
• NACRES: NA.22

特性

• 製品名: Oxyma Pure, Novabiochem^®
• 品質水準: 300
• 製品種目: Novabiochem^®
• アッセイ: ≥99.0% (HPLC)
• フォーム: crystalline powder
• 反応適合性: reaction type: Coupling Reactions
• メーカー／製品名: Novabiochem^®
• mp: 130-132 °C
• アプリケーション: peptide synthesis
• 保管温度: 2-8°C
• InChI: 1S/C5H6N2O3/c1-2-10-5(8)4(3-6)7-9/h9H,2H2,1H3/b7-4+
• InChI Key: LCFXLZAXGXOXAP-QPJJXVBHSA-N

詳細

詳細

Oxyma Pure (Ethyl cyano(hydroxyimino)acetate) is a non-explosive alternative to the canonical peptide coupling additive N-hydroxybenzotriazole (HOBt). When used in place of HOBt in carbodiimide-mediated peptide bond formation, it provides products in higher yields with less racemization. The combination of Oxyma Pure/diisopropylcarbodiimide (DIC) has proven to be particularly effective in microwave-assisted peptide synthesis.
The generation of toxic hydrogen cyanide (HCN) from the reaction between Oxyma Pure and DIC has been observed. Fortunately, this side reaction can be eliminated by substituting DIC for the more hindered t-butylethylcarbodiimide.

Associated Protocols and Technical Articles
Guide to Selection of Coupling Reagents

Literature references:
^[1] R. Subirós-Funosas, et al. (2009) Chem. Eur. J., 15, 9394
^[2] J. Collins , et al. (2014) Org . Lett ., 16, 940.
^[3] A. D. McFarland (2019) Org. Process Res. Dev., 23, 2099.
^[4] S. R. Manne, et al (2022) Org. Process Res. Dev., 26, 2894.

アプリケーション

Further applications of Oxyma Pure:

• Use in the synthesis of difficult peptides.
• As an acidic modifier to prevent aspartimide formation.

特徴および利点

• Can be used in place of HOBt in carbodiimide-mediated coupling reactions without change of protocol
• It gives results comparable to HOAt in step-wise solid-phase synthesis
• Less epimerization than HOBt in fragment condensation reactions

アナリシスノート

Color (visual): white to slight yellow to beige
Appearance of substance (visual): crystalline powder
Assay (HPLC, area%): ≥ 99.0 % (a/a)
Identity (IR): passes test
Solubility (12,5 mmol in 25 ml DMF): clearly soluble

法的情報

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable