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由来生物
mouse
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
SY38, monoclonal
化学種の反応性
bovine, rat, avian, fish, mouse, human
メーカー／製品名
Chemicon®
テクニック
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
アイソタイプ
IgG1κ
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... SYP(6855)
詳細
Synaptophysin is a 38 kDa glycoprotein present in the membrane of neuronal presynaptic vesicles in brain, spinal cord, retina, vesicles of adrenal medulla, neuromuscular junctions, and endocrine cells. It is also expressed by neuroendocrine cells throughout the body, both normal and neoplastic. Synaptophysin is a useful marker for the identification of normal neuroendocrine cells and neuroendocrine neoplasms. This antibody reacts with neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas, and gastointestinal mucosa. It also reacts with a wide spectrum of neuroendocrine neoplasms of neural type including neuroblastomas, ganglioneuroblastomas, ganglioneuromas, pheochromocytomas, chromaffin, and non chromaffin paragangliomas.
特異性
The antibody reacts with presynaptic vesicles of cerebral and spinal neurons, of neuromuscular endplates and with the retina of man, cow, rat and mouse. The antibody also reacts with vesicles of adrenal medulla and islet cells, and additionally allows specific staining of neuronal, adrenal and neuroepithelial tumors, such as pheochromocytoma, paraganglioma, islet cell tumors (incl. Insulinoma), medullary thyroid carcinoma and diverse pulmonary and gastro-intestinal carcinoids. The antibody also stains neurosecretory vesicles of certain culture cells, e.g. of the rat cell line PC-12.
免疫原
Vesicular fraction of bovine brain. The SY38 epitope is to a pentapeptide repeat structure in the carboxy-terminal cytoplasmic tail of synaptophysin (Knaus & Betz 1990).
アプリケーション
Research Category
ニューロサイエンス
ニューロサイエンス
Research Sub Category
シナプス及びシナプス生物学
シナプス及びシナプス生物学
Detect Synaptophysin using this Anti-Synaptophysin Antibody, clone SY38 validated for use in FC, IC, IH & WB.
Flow Cytometry (FACS):
A previous lot of this antibody was used in Flow Cytometry.
Western Blot:
(Provoda, 2000) Reacts with a 38 kDa transmembrane glycoprotein
Immunohistochemistry:
For relatively cytoplasm-rich neuroendocrinic tumors a final concentration of 1 µg/mL is recommended. Concerning cytoplasm deficient tumors a concentration of 2 µg/mL should be used. Ideal frozen sections (4-5 mm) are obtained from shock-frozen tissue samples. The frozen sections are air-dried and then fixed with acetone for 5-10 min at -20°C. Excess acetone is allowed to evaporate at room temperature. Material fixed in alcohol or formalin and embedded in paraffin can also be used.
It is advantageous to block unspecific binding sites by overlaying the sections with fetal calf serum for 20-30 min at room temperature. Excess of fetal calf serum is removed by decanting before application of the anti-body solution.
Cytocentrifuge preparations of single cells or cell smears are also fixed in acetone. These preparations should but should be followed directly by antibody treatment.
Optimal working dilutions must be determined by end user.
A previous lot of this antibody was used in Flow Cytometry.
Western Blot:
(Provoda, 2000) Reacts with a 38 kDa transmembrane glycoprotein
Immunohistochemistry:
For relatively cytoplasm-rich neuroendocrinic tumors a final concentration of 1 µg/mL is recommended. Concerning cytoplasm deficient tumors a concentration of 2 µg/mL should be used. Ideal frozen sections (4-5 mm) are obtained from shock-frozen tissue samples. The frozen sections are air-dried and then fixed with acetone for 5-10 min at -20°C. Excess acetone is allowed to evaporate at room temperature. Material fixed in alcohol or formalin and embedded in paraffin can also be used.
It is advantageous to block unspecific binding sites by overlaying the sections with fetal calf serum for 20-30 min at room temperature. Excess of fetal calf serum is removed by decanting before application of the anti-body solution.
Cytocentrifuge preparations of single cells or cell smears are also fixed in acetone. These preparations should but should be followed directly by antibody treatment.
Optimal working dilutions must be determined by end user.
品質
Routinely evaluated by Western Blot on mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected synaptophysin on 10 μg of mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected synaptophysin on 10 μg of mouse brain lysates.
ターゲットの説明
38 kDa
関連事項
Replaces: 04-1019
物理的形状
Protein A purified
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing 20 mM sodium phosphate buffer, 0.25 M NaCl, containing 0.1% sodium azide.
保管および安定性
Stable for 6 months at 2-8ºC from date of receipt. DO NOT FREEZE.
アナリシスノート
Control
Positive Control: Pancreas tissue
Negative Control: Normal mouse serum
Rat hippocampus tissue, mouse brain lysate.
Positive Control: Pancreas tissue
Negative Control: Normal mouse serum
Rat hippocampus tissue, mouse brain lysate.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MAB5258-20UG:
MAB5258-50UG:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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