MABE148

Anti-HMGB1 Antibody, clone HMG1-5H6

clone HMG1-5H6, from hamster

• 別名: High mobility group protein 1, Sulfoglucuronyl carbohydrate binding protein, high mobility group box 1, high-mobility group (nonhistone chromosomal) protein 1, high-mobility group box 1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: HMG1-5H6, monoclonal
• application: FACS; IF; WB
• 化学種の反応性: rat, mouse, human
• テクニック: flow cytometry: suitable; immunofluorescence: suitable; western blot: suitable
• citations: 1

特性

• 由来生物: hamster
• 品質水準: 100; 300
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: HMG1-5H6, monoclonal
• 化学種の反応性: rat, mouse, human
• テクニック: flow cytometry: suitable; immunofluorescence: suitable; western blot: suitable
• NCBIアクセッション番号: NP_034569
• UniProtアクセッション番号: P09429
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... HMGB1(3146)

詳細

詳細

HMG-1 (High-Mobility-Group protein, amphoterin) is a highly conserved protein with more than 95% amino acid identity between rodent and human HMG-1. It is a prevalent non-histone chromatin component and a non-sequence specific DNA binding protein. HMG-1 consists of two homologous HMG boxes rich in basic amino acids and an acidic tail at the carboxy-terminus. HMG-1 is involved in the regulation of chromatin structure as well as being involved, either as positive or negative factors with various aspects of DNA replication, transcription, repair, and ligation. HMG-1, identified as a membrane associated protein termed “amphoterin,” mediates neurite outgrowth, tumor outgrowth, and metastasis. It participates in plasminogen activation and is recognized as a late mediator of endotoxin lethality in mice.

免疫原

Recombinant protein corresponding to mouse HMGB1.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

エピジェネティクス及び核内機能分子

Research Sub Category
エピジェネティクス

細胞周期、DNA複製及び修復

Immunofluorescence Analysis: 10 µg/mL from a representative lot detected HMGB1 in NIH/3T3 cells. (Image courtesy of Dr. Hideo Yagita, Juntendo University School of Medicine.)

Flow Cytometry Analysis: A representative lot detected HMGB1 in RAW264.7 cells treated with Cytofix/Cytoperm. (Image courtesy of Dr. Hideo Yagita, Juntendo University School of Medicine.)

Use Anti-HMGB1 Antibody, clone HMG1-5H6 (Hamster Monoclonal Antibody) validated in WB, IF, FC to detect HMGB1 also known as High-mobility group box 1.

品質

Evaluated by Western Blot in NIH/3T3 cell lysate.

Western Blot Analysis: 1:500 dilution of this antibody detected HMGB1 on 10 µg of NIH/3T3 cell lysate.

ターゲットの説明

~ 30 kDa observed

物理的形状

Protein G

Format: Purified

Purified hamster monoclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
NIH/3T3 cell lysate

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable