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由来生物
bovine (calf) intestine
品質水準
形状
solution
比活性
~3,000 U/mg enzyme protein (+37°C, 4-nitrophenyl phosphate substrate, diethanolamine as buffer)
包装
pkg of 1.5 mL (15 mg)
メーカー／製品名
Roche
最適pH
9.8
pH範囲
9.0-11.0
輸送温度
wet ice
詳細
Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
Amino groups: 8-16 moles/mole enzyme
Amino groups: 8-16 moles/mole enzyme
アプリケーション
Alkaline phosphatase can be coupled to other proteins via its amino groups. The enzyme solution can be used directly for conjugation without prior dialysis.
品質
Purity: single peak (HPLC)
シーケンス
Purification procedure
AP from intestine is a dimeric glycoprotein, it is not known, whether the carbohydrate part is separated during purification procedure. Content of amino groupsIn the literature the carbohydrate content is 2 to 20 percent so one should find approx. 70 amino groups/mol.
In addition one researcher determined the amino groups and found 50 to 60 lysins/mol.-SH groups in AP
No information in literature about -SH groups in AP.
Carbohydrate structure
Results of investigation of the carbohydrate structure of several lots of alkaline phosphatase are available.
NH2- and carbohydrate estimation
Results of NH2- and carbohydrate estimation are available.
AP from intestine is a dimeric glycoprotein, it is not known, whether the carbohydrate part is separated during purification procedure. Content of amino groupsIn the literature the carbohydrate content is 2 to 20 percent so one should find approx. 70 amino groups/mol.
In addition one researcher determined the amino groups and found 50 to 60 lysins/mol.-SH groups in AP
No information in literature about -SH groups in AP.
Carbohydrate structure
Results of investigation of the carbohydrate structure of several lots of alkaline phosphatase are available.
NH2- and carbohydrate estimation
Results of NH2- and carbohydrate estimation are available.
単位の定義
Unit Conversion: Approx. 4.35 U (EIA grade AP) [37 °C, 4-NPP as substrate, diethanolamine as buffer, pH 9.8] = 1 U [+25 °C, 4-NPP as substrate, glycine as buffer, pH 10.5]
1.0 U [+37 °C, QC assay conditions] = 0.73 U [+30 °C, QC assay conditions] = 0.48 U [+25 °C, QC assay conditions]
1.0 U [+37 °C, QC assay conditions] = 0.73 U [+30 °C, QC assay conditions] = 0.48 U [+25 °C, QC assay conditions]
物理的形状
Solution, ready-to-use, 10mg/ml
調製ノート
Storage conditions (working solution): The diluted enzyme (1:10 in 3 M NaCl) will give a stability of 6 months at 4 °C.
1:10 dilution in water will give stability for 1 week at 4°C.
Do not freeze the diluted or undiluted enzyme, because freezing will destroy enzyme activity.
1:10 dilution in water will give stability for 1 week at 4°C.
Do not freeze the diluted or undiluted enzyme, because freezing will destroy enzyme activity.
保管および安定性
Store at 2 to 8 °C. (Do not freeze)
その他情報
For life science research only. Not for use in diagnostic procedures.
WGK
WGK 1
引火点(°F)
does not flash
引火点(℃)
does not flash
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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プロトコル
Alkaline phosphatase (AP) is a non-specific phosphomonoester hydrolase that catalyzes the hydrolysis of a wide variety of organic monophosphates.
アルカリホスファターゼ（AP）は、幅広い種類の有機一リン酸の加水分解を触媒する非特異的リン酸モノエステル加水分解酵素です。
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