A2556

モノクロナール抗ウサギIgG (γ-鎖特異的)

clone RG-96, purified immunoglobulin, buffered aqueous glycerol solution

• 別名: Monoclonal Anti-Rabbit IgG (γ-chain specific)
• UNSPSCコード: 12352203
• NACRES: NA.46

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: alkaline phosphatase conjugate
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: secondary antibodies
• クローン: RG-96, monoclonal
• 形状: buffered aqueous glycerol solution
• 化学種の反応性: rabbit
• 以下との反応禁止: goat, feline, pig, guinea pig, rat, bovine, canine, human, horse, sheep, chicken
• テクニック: direct ELISA: 1:50,000; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100; western blot: 1:200,000-1:400,000 using total cell extract of HeLa cells
• アイソタイプ: IgG1
• 輸送温度: wet ice
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

本製品はウサギIgGのγ（重）鎖に位置するエピトープを認識します。イムノブロットでは、本抗体は未変性および変性したウサギIgGを認識します。ELISAでは、本抗体はウサギIgGに特異的に反応しますが、ウサギIgAおよびIgMまたはヒトIgG、IgAおよびIgMとは交差反応しません。以下の生物種のIgGとも交差反応しません：ウシ、ネコ、ニワトリ、イヌ、ヤギ、モルモット、ウマ、ブタ、ラットまたはヒツジ。

Monoclonal Anti-Rabbit IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein and mainly helps in immune defense. IgG is a major class of immunoglobulin. Rabbit IgG is further divided into five classes- IgM, IgG, IgA, IgD and IgE.

アプリケーション

Monoclonal Anti-Rabbit IgG (γ-chain specific)Alkaline Phosphatase antibody produced in mouse has been used in enzyme-linked immunosorbent assay (ELISA) and immunoblotting.

生物化学的／生理学的作用

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

物理的形状

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl₂, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)