A8481

モノクロナール抗γ-アクチン

clone 2-2.1.14.17, purified from hybridoma cell culture

• 別名: Anti-ACTG1
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: 2-2.1.14.17, monoclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~43 kDa
• 化学種の反応性: mouse, chicken, canine, bovine, human, hamster
• 包装: antibody small pack of 25 μL
• 濃度: ~2 mg/mL
• テクニック: immunohistochemistry: suitable; indirect ELISA: suitable; western blot: 0.25-0.5 μg/mL using total cell extract of 3T3 cells
• アイソタイプ: IgG1
• UniProtアクセッション番号: P63261
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: bovine ... ACTG1(404122) ; chicken ... ACTG1(415296) ; human ... ACTG1(71) ; mouse ... Actg1(11465)

詳細

詳細

γ-Actin is mainly present in sarcolemma and to a lesser extent in reticular lattice of normal muscles. Increased levels of γ-actin have been linked to dystrophin loss and subsequent cell signaling and gene expression defects.

Monoclonal Anti-γ-Actin (mouse IgG1 isotype) is derived from the hybridoma 2-2.1.14.17 produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice. Actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms. Actin isoforms show more than 90% overall sequence homology, but only 50-60% homology in their 18 NH₂-terminal residues. Actin constitutes for around 15% of the total protein. It exists in globular and filamentous forms.

特異性

Monoclonal Anti-γ-Actin recognizes human, bovine, canine, mouse, hamster and chicken γ-actin.

アプリケーション

Anti-γ-Actin antibody, Mouse monoclonal has been used in :

• immunofluorescence
• enzyme linked immunosorbent assay (ELISA)
• western blotting
• immunohistochemistry

Mouse monoclonal Anti-γ-Actin antibody has been used for immunofluorescence assays. The antibody can also be used for ELISA, IHC and western blot assays.

Protein lysates from human Jurkat T-leukemic cells were subjected to western blot and probed using monoclonal mouse anti-actin (AC-40) to ensure equal loading.

生物化学的／生理学的作用

The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin is significantly involved in the microfilament system. It participates in muscle contraction and gene regulation. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Increased levels of γ-actin have been linked to dystrophin loss and subsequent cell signaling and gene expression defects.

物理的形状

15 mMアジ化ナトリウム含有のpH 7.4の0.01 Mリン酸緩衝食塩水溶液.

その他情報

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)