コンテンツへスキップ
Merck

C7886

Sigma-Aldrich

クレアチンホスホキナーゼ ウシ心臓由来

Type III, salt-free, lyophilized powder, ≥30 units/mg protein

別名:

ATP:クレアチン N-ホスホトランスフェラーゼ, CPK, クレアチンキナーゼ, ホスホクレアチンホスホキナーゼ

ログイン組織・契約価格を表示する


About This Item

CAS番号:
Enzyme Commission number:
EC Number:
MDL番号:
UNSPSCコード:
12352204
eCl@ss:
32160410
NACRES:
NA.54

由来生物

bovine heart

品質水準

詳細

Contains primarily isozyme MM

タイプ

Type III

フォーム

salt-free, lyophilized powder

比活性

≥30 units/mg protein

分子量

80 kDa

保管温度

−20°C

詳細

Research area: Cell Signaling
Creatine phosphokinase (CPK) is an intracellular enzyme found predominantly in skeletal muscle, brain, and myocardium. CPK consists of two polypeptide subunits, namely M (muscle type) and B (brain type). The lighter subunit is present in larger amounts. The genes encoding these subunits are on distinct chromosomes, with B on 14q32 and M on 19q13. These subunits facilitate the development of three tissue-specific isoenzymes: CPK-MB (cardiac muscle), CPK-MM (skeletal muscle), and CPK-BB (brain).

アプリケーション

Creatine Phosphokinase from bovine heart has been used:

  • in the reaction mix for the import of in vitro synthesized wild-type FLAG/MYC-tagged LYR Motif Containing 7 (LYRM7-F/M) or the LFK tripeptide replaced with alanine (LFK-AAA) mutant into isolated mitochondria
  • in autoubiquitination assay
  • in the preparation of premix buffer for adenylyl cyclase activity
  • in the preparation of E-mix for in vitro nuclear assembly and isolation
  • in in vitro vesicular fusion assay.
  • in ATP regeneration system, to facilitate the reactivation of nonmotile axonemes.
  • in subcellular in vitro fusion assay of autophagosome with lysosome.
  • in in vitro protein translation assay.
  • for tATPase assay of myofibrillar protein isolated from rabbit. This assay evaluated the kinetic influence of bound creatine kinase (CK) on Ca2+-activated myosin ATPase.
  • for the enzymatic hydrolysis of protein samples during tryptophan estimation by pyrolysis gas chromatography.
Creatine phosphokinase from bovine heart has been used to investigate whether endothelial cell growth is stimulated by ischemic hearts. Creatine phosphokinase from bovine heart has also been used to evaluate the effect of high but nontoxic dietary intake of copper and selenium on metabolism in calves.

生物化学的/生理学的作用

Creatine kinase plays a key role in the energy metabolism of cells with intermittently high and fluctuating energy requirements. Examples of such cells include cardiac or skeletal muscle cells and neural tissues of brain and retina. The enzyme catalyzes the reversible transfer of the phosphoryl group from phosphorylcreatine to ADP, in order to generate ATP. The molecular mass of the protein is found to be approximately 80,000 Da. It is made up of 2 subunits, each having a molecular weight of 40,000 ± 2000. The lighter subunit is present in larger amounts.CPK is released from the cellular cytosol into the systemic circulation due to disruption of cell membranes caused by hypoxia or other injuries. Furthermore, it serves as a diagnostic indicator for rhabdomyolysis and acute myocardial infarction (AMI), along with other medical disorders such as acute muscle injury, arrhythmias, and congestive cardiac failure.

注意

希釈による不活性化を避けるため、反応バッファ-には0.1%アルブミンを添加することをお勧めします。

単位の定義

1ユニットは、pH 7.4、30°C、1分間で、1.0 μmolのリン酸をホスホクレアチニンからADPへと転移させる酵素量です。

アナリシスノート

タンパク質はBiuret法で測定。

保管分類コード

11 - Combustible Solids

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable

個人用保護具 (PPE)

Eyeshields, Gloves, type N95 (US)


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

C7886-500UN:
C7886-3.5KU:
C7886-1KU:
C7886-VAR:
C7886-35KU:
C7886-17.5KU:
C7886-BULK:


最新バージョンのいずれかを選択してください:

試験成績書(COA)

Lot/Batch Number

適切なバージョンが見つかりませんか。

特定のバージョンが必要な場合は、ロット番号またはバッチ番号で特定の証明書を検索できます。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

P J Russell et al.
Clinical chemistry, 30(9), 1555-1557 (1984-09-01)
Elemental sulfur is a specific and potent inhibitor of the muscle-type isoenzyme of adenylate kinase (EC 2.7.4.3). We find inhibition by sulfur and by diadenosine pentaphosphate to be similarly potent and specific. Some properties of inhibition of adenylate kinase isoenzymes
Internal friction controls active ciliary oscillations near the instability threshold
Mondal D, et al.
Science Advances, 6(33), eabb0503-eabb0503 (2020)
Mucoricin is a Ricin-Like Toxin that is Critical for the Pathogenesis of Mucormycosis
Soliman SSM, et al.
Nature microbiology, 6(3), 313?326-313?326 (2021)
Cholesterol impairs autophagy-mediated clearance of amyloid beta while promoting its secretion
Barbero-Camps E, et al.
Autophagy, 14(7), 1129?1154-1129?1154 (2018)
NRBF2 is a RAB7 effector required for autophagosome maturation and mediates the association of APP-CTFs with active form of RAB7 for degradation
Cai CZ, et al.
Autophagy, 17(5), 1112?1130-1112?1130 (2021)

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

製品に関するお問い合わせはこちら(テクニカルサービス)