M4642

イーグル最少必須培地

With Hanks′ salts and ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture

• 別名: MEM
• UNSPSCコード: 12352207
• NACRES: NA.75

特性

• 製品名: イーグル最少必須培地, With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
• フォーム: powder
• テクニック: cell culture | mammalian: suitable
• 構成: glucose: yes; Hanks’ salts (2% CO₂): yes; L-glutamine: yes; Earle’s salts (5% CO₂): no; NaHCO₃: no; phenol red: yes; sodium pyruvate: no; stable glutamine: no; HEPES: no
• 輸送温度: ambient
• 保管温度: 2-8°C

詳細

詳細

Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has high concentrations of amino acids, so the medium closely approximates the protein concentration of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has increased the applicability of this medium. This medium lacks calcium that permits the growth of cells in suspension. Modification of the original MEM formulation. Earle′s salts have been replaced with Hanks′ salts. Since the bicarbonate concentration is substantially lower, this medium has a substantially reduced buffering capacity.

アプリケーション

Minimum Essential Medium Eagle (MEM) has been used:

• as a component of the Hank′s MEM (HMEM)-Pipes buffer to induce anaphylatoxin induced-ear swelling in mice
• as a component of the slice culture medium (SCM) for the incubation of mouse hippocampal slice cultures
• to culture Müller cells from guinea pigs

数量

培地1 Lあたり粉末10.6 gが含まれる組成になっています。

再構成

0.35 g/L 炭酸水素ナトリウムを添加してください。

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1