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詳細
Peroxidase (POD) is an enzyme that includes a group of specific enzymes such as nicotinamide adenine dinucleotide (NAD) peroxidase, NADP peroxidase, fatty acid peroxidase and a group of non-specific enzymes from various sources. It is present in animals, higher plants and other organisms.
アプリケーション
For direct conjugation to compounds containing free sulfhydryl groups for use as an enzyme-linked detection reagent.
Instructions for use included. In a study on horseradish peroxidase, the Maleimide compounds succinimidyl 4-maleimidobutyrate and succinimidyl 6-maleimidohexanoate have been shown to be the most effective in the conjugation of Fab′ to horseradish peroxidase. This enzyme has also been used in a study to investigate the effectiveness in staining a mitochondrial protein, lipoamide dehydrogenase.
Instructions for use included. In a study on horseradish peroxidase, the Maleimide compounds succinimidyl 4-maleimidobutyrate and succinimidyl 6-maleimidohexanoate have been shown to be the most effective in the conjugation of Fab′ to horseradish peroxidase. This enzyme has also been used in a study to investigate the effectiveness in staining a mitochondrial protein, lipoamide dehydrogenase.
To increase signal intensity of immunoconjugates, immunoglobulins were synthetically modified to contain multiple primary amines and covalently coupled to maleimide activated peroxidase. This can result in more efficient conjugation and signal amplification.
生物化学的／生理学的作用
Peroxidase (POD) helps in the dehydrogenation of several organic compounds like phenols, aromatic amines, hydroquinones etc.
その他情報
マレイミドカプロン酸N-ヒドロキシスクシンイミドエステルにより活性化したType VI ペルオキシダ-ゼ
単位の定義
One unit will form 1.0 mg of purpurogallin from pyrogallol in 20 seconds at pH 6.0 at 20°C.
物理的形状
凍結乾燥品(トレハロ-ス、クエン酸ナトリウム含有)
シグナルワード
Danger
危険有害性情報
危険有害性の分類
Resp. Sens. 1 - Skin Sens. 1
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
dust mask type N95 (US), Eyeshields, Gloves
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Biochemical Methods (1996)
S Matuda et al.
In vivo (Athens, Greece), 1(4), 221-227 (1987-07-01)
IgG-maleimide peroxidase, Fab'-maleimide peroxidase, polymer and monomer types of Fab'-periodate peroxidase were prepared from an antibody against rat lipoamide dehydrogenase, a component of the pyruvate dehydrogenase complex which is located in mitochondria. They were examined for immunohistochemical staining of the
Subhash Dhawan
Peptides, 23(12), 2091-2098 (2003-01-22)
Immunoconjugates are widely used for indirect detection of analytes (such as antibodies or antigens) in a variety of immunoassays. However, the availability of functional groups such as primary amines or free sulfhydryls in an immunoglobulin molecule is the limiting factor
S Hashida et al.
Journal of applied biochemistry, 6(1-2), 56-63 (1984-02-01)
Nine different maleimide compounds were evaluated for the conjugation of Fab' to horseradish peroxidase through thiol groups in the hinge. The compounds evaluated were succinimidyl maleimidoacetate (I), succinimidyl 4-maleimidobutyrate (II), succinimidyl 6-maleimidohexanoate (III), succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (IV), succinimidyl m-maleimidobenzoate (V), succinimidyl
Kitagawa, T., et al.
Chemical & Pharmaceutical Bulletin, 29(4), 1131-1131 (1981)
プロトコル
Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275).
2,2'-アジノ-ビス（3-エチルベンズチアゾリン-6-スルホン酸）を基質として用いたペルオキシダーゼのアッセイ手順の標準化
To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.
This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate.
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