P9249

モノクロナール抗p53−ビオチン マウス宿主抗体

~1 mg/mL, clone DO-1, ascites fluid, buffered aqueous solution

• MDL番号: MFCD01864735
• UNSPSCコード: 12352203
• NACRES: NA.41
• 結合体: biotin conjugate
• application: FACS; ICC; IHC; IP; WB
• クローン: DO-1, monoclonal
• 化学種の反応性: human
• citations: 11
• テクニック: flow cytometry: suitable; immunocytochemistry: 5-10 μg/mL using human A431; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 0.5-1 μg/mL using human A431 cell extracts

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: biotin conjugate
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: primary antibodies
• クローン: DO-1, monoclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~53 kDa
• 化学種の反応性: human
• 濃度: ~1 mg/mL
• テクニック: flow cytometry: suitable; immunocytochemistry: 5-10 μg/mL using human A431; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 0.5-1 μg/mL using human A431 cell extracts
• アイソタイプ: IgG2a
• UniProtアクセッション番号: P04637
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... TP53(7157)

詳細

詳細

p53 is a tumor suppressor gene expressed in a wide variety of tissues. It is a tetrameric nuclear DNA-binding phosphoprotein. The gene encoding it is localized on human chromosome 17p13.1 and mouse chromosome 11.

免疫原

recombinant human wild type p53. The epitope resides beween amino acids 20-25 of human p53.

アプリケーション

Monoclonal Anti-p53-Biotin antibody produced in mouse has been used for Western Blotting.

生物化学的／生理学的作用

Tumor suppressor p53 has the capability to induce cell cycle arrest and has a role in DNA repair, senescence and apoptosis. It binds to Simian vacuolating virus 40 (SV40) T-antigen and human papilloma virus E6 protein. The p53 gene is mutated in various cancers, such as of the breast, ovarian, bladder, colon and lung.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

保管および安定性

For continuous use, store at 2-8°C for up to one month.
For extended storage, freeze in working aliquots.
Repeated freezing and thawing, or storage in “frostfree”freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

その他情報

In order to obtain the best results using various techniques and preparations, we recommend determining optimal working dilutions by titration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)