SAE0198

Calprotectin, human recombinant

Stabilized heterodimer of fusion tagged S100A9 and S100A8

• 別名: MRP8/14 and S100A8/A9 complex
• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• 品質水準: 200
• アッセイ: ≥90% (SDS-PAGE)
• フォーム: lyophilized powder
• 輸送温度: wet ice
• 保管温度: −20°C

詳細

詳細

This recombinant human calprotectin is a heterodimer composed of tagged versions of both S100A8 and S100A9, where a 6xHis Sumo tag is added to the N-terminus of S100A8 and, a 6XHis tag is added to the N-terminus of S100A9. This tagged version was found to have enhanced stability as compared to untagged versions of this protein. Removal of the tags can be achieved using HRV-3C protease (SAE0045 or SAE0110).

アプリケーション

Fecal Calprotectin is often used as a biomarker to identify inflammation in gastrointestinal disease¹. This product is a recombinant version of the human protein found endogenously.

生物化学的／生理学的作用

Calprotectin is a calcium and zinc binding protein that belongs to the S100 family. It is a heterodimer protein composed of S100A8 and S100A9.¹ The protein is expressed in myeloid cells and in circulating monocytes and neutrophils, where it accounts for 60% of the cytosolic protein content. Calprotectin was found to play major roles in inflammation and the immune response by activation of NF-kB pathway via TLR4 and AGER,² and its antimicrobial activity possibly through chelating of Zn.³ High concentrations of calprotectin are found and serve as a biomarker in rheumatoid, cystic fibrosis, inflammatory bowel disease and in Crohn′s disease.⁴ A meta-analysis also identified calprotectin as a potential biomarker for COVID-19.⁵

物理的形状

The product is lyophilized from a sterile 0.2 µm filtered solution containing 12.5 mM Tris-Cl (pH 8.0), 150 mM NaCl, and 1 mM DTT.

調製ノート

Reconstitute lyophilized vial in DMEM or in PBS to a concentration up to 500 µg/mL. Spin down to collect material. Reconstituted product should be stored in working aliquots at –20 °C. Do not store in a frost-free freezer. Avoid freeze-thaw cycles.

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H319
• 注意書き: P264 - P280 - P305 + P351 + P338 - P337 + P313
• 危険有害性の分類: Eye Irrit. 2
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1