SRP3070

IGF-II ヒト

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• 別名: インスリン様増殖因子-II
• UNSPSCコード: 12352202
• NACRES: NA.32

特性

• 由来生物: human
• リコンビナント: expressed in E. coli
• アッセイ: ≥98% (HPLC); ≥98% (SDS-PAGE)
• フォーム: lyophilized
• 有効性: ≤2.0 ng/mL ED₅₀
• 分子量: 7.5 kDa
• 包装: pkg of 50 μg
• テクニック: cell culture | mammalian: suitable
• 不純物: <0.1 EU/μg endotoxin, tested
• 色: off-white to yellow
• UniProtアクセッション番号: P01344
• 輸送温度: wet ice
• 保管温度: −20°C
• 遺伝子情報: human ... IGF2(3481)

詳細

詳細

Insulin-like growth factors (IGFs) are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the insulin gene family, which also contains insulin and relaxin. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but, IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions. Recombinant human IGF-I and IGF-II are globular proteins containing 70 and 67 amino acids, respectively, and three intra-molecular disulfide bonds.

アプリケーション

IL-4 from mouse has been used to activate B-cells.

生物化学的／生理学的作用

The insulin-like growth factors (IGFs) are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro.

シーケンス

AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE

物理的形状

凍結乾燥品(添加物不含)。

再構成

バイアルは、開封前に遠心してください。水で濃度0.1～1.0 mg/mLに復元してください。ボルテックスは行なわないでください。水溶液は2～8°Cで最長1週間保存できます。長期保存には、担体タンパク質(0.1% BSAなど)含有バッファーでさらに希釈し、1回の使用量ずつに分注して-20～-80°Cで保存することを推奨します。

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable