T2824

モノクロナール抗腫瘍壊死因子-α ハムスター宿主抗体

~2 mg/mL, clone TN3-19.12, purified immunoglobulin, buffered aqueous solution

• 別名: 抗TNF-α抗体
• MDL番号: MFCD01632994
• UNSPSCコード: 51111800
• NACRES: NA.41

特性

• 由来生物: hamster
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: TN3-19.12, monoclonal
• フォーム: buffered aqueous solution
• 化学種の反応性: rat, mouse
• 包装: antibody small pack of 25 μL
• 濃度: ~2 mg/mL
• テクニック: immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; neutralization: suitable; western blot: 0.25-0.5 μg/mL using recombinant mouse TNF-α
• UniProtアクセッション番号: P16599
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Tnf(21926) ; rat ... Tnf(24835)

詳細

詳細

Monoclonal Anti-Mouse Tumor Necrosis Factor-α (TNF-α) is derived from the TN3-19.12 hybridoma produced by the fusion of mouse myeloma cells (P3X63-Ag8.653) and splenocytes from male Armenian hamster immunized with purified mouse recombinant TNF-α. Tumor necrosis factor (TNF) protein family contains two members: TNF-α (also known as cachectin) and TNF-β (also known as lymphotoxin). These proteins are small molecules with a molecular weight of 17 and 25 kDa respectively and share 30% amino acid homology. TNF-α and -β are secreted from many different cells such as lymphocytes, neutrophils, and macrophages.

特異性

Mouse monoclonal clone TN3-19.12 anti-Tumor Necrosis Factor-α antibody recognizes mouse and rat TNF-α (approx. 17 kDa in its monomer form and 51 kDa in its homotrimer form) and cross-reacts with mouse TNF-β.

免疫原

精製済み組み換えマウスTNF-α。

アプリケーション

Monoclonal Anti-Tumor Necrosis Factor-α antibody produced in hamster has been used in:

• immunoblotting
• enzyme-linked immunosorbent assay (ELISA)
• immunoprecipitation
• neutralization

生物化学的／生理学的作用

TNF-α is a major mediator of inflammatory responses and its over-production results in extensive tissue damage associated with endotoxemia and cachexia. High serum levels of TNF are associated with septic shock, rejection of renal transplants, parasitic infections, and various neoplastic diseases. TNF-α exhibits growth-enhancing activities for normal cells like fibroblasts, T and B cells, activation of endothelial cells, and differentiation of myeloid cells. In neoplastic cells, TNF-α exerts growth inhibitory activities that can be either cytostatic or cytotoxic.

物理的形状

0.01M PBS溶液(0.2μmフィルタ-濾過済み, pH 7.4)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable