581301-U

Ascentis^® C18 (3 µm) HPLC Columns

L × I.D. 10 cm × 2.1 mm, HPLC Column

• 別名: Ascentis RP18 HPLC Column
• UNSPSCコード: 41115700
• eCl@ss: 32110501
• NACRES: SB.52
• L × 内径: 10 cm × 2.1 mm
• 表面積: 450 m²/g
• 粒径: 3 μm
• マトリックス活性基: C18 (octadecyl) phase
• ポアサイズ: 100 Å
• Matrix: fully porous particle; silica gel high purity, spherical

特性

• 製品名: Ascentis^® C18 HPLCカラム, 3 μm particle size, L × I.D. 10 cm × 2.1 mm
• 物質: stainless steel column
• 品質水準: 100
• 認証: suitable for USP L1 (Similar to Phenomenex Luna C18)
• 製品種目: Ascentis^®
• 特徴: endcapped
• メーカー／製品名: Ascentis^®
• 包装: 1 ea of
• 標識化の程度: 25% Carbon loading
• パラメーター: ≤70 °C temp. range; 400 bar pressure (5801 psi)
• テクニック: HPLC: suitable; LC/MS: suitable
• L × 内径: 10 cm × 2.1 mm
• 表面積: 450 m²/g
• 表面被覆率: 3.7 μmol/m²
• 不純物: <5 ppm metals
• Matrix: fully porous particle; silica gel high purity, spherical
• マトリックス活性基: C18 (octadecyl) phase
• 粒径: 3 μm
• ポアサイズ: 100 Å
• 動作pH範囲: 2-8
• アプリケーション: food and beverages
• 分離法: reversed phase

詳細

詳細

AscentisカラムファミリーはSupelcoの科学者達が開発した第4世代のHPLCカラム技術です。Ascentisは孔径100Åの高純度シリカ(粒径は3、5、10 μm)に官能基を結合させています。カラムは低分子用として設計されており、マイクロカラム(内径1.0 mm)から調製用(内径50 mm)まで揃っています。ファミリーにはC18、C8、Phenyl、Siに加えて、極性基導入型であるRP-Amideがあります。

Ascentis C18は、検出しにくい化合物に対しても、対称的なピーク形状と優れた保持時間を示す、非常に安定した信頼性の高いHPLCカラムの第一候補です。

アプリケーション

• Identification of recombinant human insulin and biosynthetic insulin analogues by multiplexed targeted unlabeled mass spectrometry of proteotypic tryptic peptides.: This study employs the Ascentis^® C18 HPLC column for the separation of proteotypic tryptic peptides, allowing the identification of human insulin and biosynthetic insulin analogues through multiplexed targeted unlabeled mass spectrometry. The method demonstrated high specificity and sensitivity, enhancing the accuracy of insulin analysis in pharmaceutical research (Qasem et al., 2019).


• Development and validation of a novel stability-indicating HPLC method for the simultaneous assay of betamethasone-17-valerate, fusidic acid, potassium sorbate, methylparaben, and propylparaben in a topical cream preparation.: Utilizing the Ascentis^® C18 HPLC column, this research presents a novel stability-indicating method for the simultaneous assay of multiple compounds in a topical cream. The validated method offers robust stability assessment, critical for ensuring the quality and efficacy of pharmaceutical formulations (Byrne et al., 2014).


• HPLC-DAD and HPLC-ESI-MS/MS methods for metabolite profiling of propolis extracts.: This paper describes the use of the Ascentis^® C18 HPLC column in HPLC-DAD and HPLC-ESI-MS/MS methods to profile metabolites in propolis extracts. The column′s performance in separating complex mixtures highlights its utility in natural product research and quality control (Pellati et al., 2011).


• Practical comparison of 2.7 microm fused-core silica particles and porous sub-2 microm particles for fast separations in pharmaceutical process development.: This study compares the performance of the Ascentis^® C18 HPLC column with other chromatographic columns for fast separations in pharmaceutical process development. The Ascentis^® column provided superior separation efficiency and speed, making it advantageous for high-throughput pharmaceutical analysis (Abrahim et al., 2010).

特徴および利点

• 優れた保持率
• 対称性高いピ-ク形状
• 高い再現性
• 完璧なLC-MS適合性

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法的情報

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable