추천 제품
ligand
iminodiacetic acid
Quality Level
설명
for metal affinity chromatography (IMAC), suspension in 20% ethanol and 150 mM NaCl (40-90 µm)
무균
sterile (Caustic Stable)
양식
resin
정제법
immobilized metal affinity chromatography (IMAC)
제조업체/상표
Fractogel®
파라미터
8 bar max. pressure
~240 cm/hr flow rate
기질 활성군
methacrylate
폭 넒음
40-90 μm
용량
~60 mg binding capacity( lysozyme/ml of resin)
전이 온도
flash point 35 °C (calculated)
응용 분야
recombinant protein
분리 기술
affinity
저장 온도
2-30°C
일반 설명
Fractogel® EMD Chelate combines our tentacle structure with iminodiacetic acid as the metal chelating ligand
특징 및 장점
Fractogel® EMD Chelate (M) enables users to:
- Efficiently purify histidine-tagged recombinant proteins
- Coordinate metal ions to non-bonding electron pairs of the amino acid side chains and separate peptides
- Ensure timely and successful startup and operation
- Minimize hydrophobic interactions due to the tentacles technology, resulting in superior selectivity and recovery
- Handle multiple CIP and packing cycles with the chemically and physically robust bead, leading to a longer resin lifetime
포장
- 1.10338.0100: Fractogel® EMD Chelate (M) Resin 100ml
- 1.10338.0010: Fractogel® EMD Chelate (M) Resin 10 ml
- 1.10338.0500: Fractogel® EMD Chelate (M) Resin 500 ml
- 1.10338.5000: Fractogel® EMD Chelate (M) Resin 5 L
Plastic bottle
분석 메모
Appearance: Milky tubid suspension, free from impurities (foreigen particles)
Microscopic evaluation: Uniform spherical particles, no agglomerates, no fines
Cerium: ≤ 10 µg/g
Copper-(II)-binding capacity: 60 - 100 µmol/ml
Extractable matter (water): ≤ 0.05 %
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1.0 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Functional test: passes test
Functional test: Separation of chymotrypsinogen A and myoglobin
Microscopic evaluation: Uniform spherical particles, no agglomerates, no fines
Cerium: ≤ 10 µg/g
Copper-(II)-binding capacity: 60 - 100 µmol/ml
Extractable matter (water): ≤ 0.05 %
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1.0 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Functional test: passes test
Functional test: Separation of chymotrypsinogen A and myoglobin
법적 정보
FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany
신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Flam. Liq. 3
Storage Class Code
3 - Flammable liquids
WGK
WGK 1
Flash Point (°F)
95.0 °F
Flash Point (°C)
35 °C
시험 성적서(COA)
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