추천 제품
생물학적 소스
mouse
Quality Level
재조합
expressed in E. coli
분석
>95% (SDS-PAGE)
양식
solution
효능
<0.5 ng/mL EC50
분자량
17,200 Da
포장
pkg of 10,000 U (5 μg, 1 ml)
제조업체/상표
Roche
저장 조건
avoid repeated freeze/thaw cycles
기술
inhibition assay: suitable
불순물
<0.1 EU/μgtested (LAL test)
UniProt 수납 번호
저장 온도
−20°C
유전자 정보
mouse ... Il2(16183)
일반 설명
Contents: 10,000 U/ml solution in PBS and 1 mg/ml BSA, filtered through a 0.2 μm pore size membrane
Interleukin-2 (IL-2, also known as T-cell growth factor, TCGF) is a lymphokine produced by lectin- or antigen-activated T-cells. It plays an important immunoregulatory role. This factor was first identified by its ability to promote the long-term in vitro proliferation of activated T-cells. It also promotes the generation and proliferation of cytotoxic T-cells, natural killer (NK) cells, and lymphokine-activated killer (LAK) cells. IL-2 also induces other lymphokines such as interferon-γ and B-cell growth factor (BCGF-1).
Recombinant Interleukin-2, mouse (mIL-2), is produced in E. coli and purified by standard chromatographic methods.
특이성
Mouse IL-2 is effective on mouse cells, but not on human cells.
애플리케이션
Recombinant murine IL-2 is produced in E. coli and exhibits the following:
- Supports the growth of murine CTLL cells (murine T cell line), but not that of human T-cells.
- Strongly inhibits the binding of recombinant human IL-2 to murine responder cells.
- Weakly inhibits the binding to human responder cells.
- Shares identical biological and immunological activities with human IL-2.
- Is a convenient tool for extensive studies of the pharmacological and physiological activities of IL-2 in murine models.
- used for the stimulation of murine T-cells
품질
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)
서열
Chain Length 149 AA
One polypeptide chain (149 amino acids), identical to natural mouse IL-2, but not glycosylated. Glycosylation is not essential for biological activity.
단위 정의
EC50 definition: The amount of mIL-2 that is required to support half-maximal stimulation of cell proliferation (XTT cleavage) with CTLL-2 cells (1 unit equals ≤0.5 ng).
제조 메모
Working solution: Dilute the concentrated IL-2 solution (10,000 U/ml) with PBS or culture medium containing 1 mg/ml BSA [or HSA (human serum alburnin)], or 1 to 10% serum (v/v).
Storage conditions (working solution): -15 to -25 °C
Note: Avoid repeated freezing and thawing.
Storage conditions (working solution): -15 to -25 °C
Note: Avoid repeated freezing and thawing.
기타 정보
Specific activity/EC 50: >2 x 106 U/mg, <0.5 ng/ml (hIL-2, NIBSC, 1st international standard, 86/504), at least the same specific activity (EC50) compared to the indicated standard is guaranteed.
Note: The biological activity of this product may vary in different in vitro applications. Determine the optimal concentration range for specific applications.
EC 50 definition/Unit definition: The amount of mIL-2 that is required to support half-maximal stimulation of cell proliferation (XTT cleavage) with CTLL-2 cells (1 unit equals =0.5 ng).
Note: The biological activity of this product may vary in different in vitro applications. Determine the optimal concentration range for specific applications.
EC 50 definition/Unit definition: The amount of mIL-2 that is required to support half-maximal stimulation of cell proliferation (XTT cleavage) with CTLL-2 cells (1 unit equals =0.5 ng).
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point (°F)
No data available
Flash Point (°C)
No data available
이미 열람한 고객
James Lee et al.
Methods in molecular biology (Clifton, N.J.), 506, 83-96 (2008-12-27)
In comparison to human T cells, efficient retroviral gene transfer and subsequent expansion of murine primary T cells is more difficult to achieve. Herein, we describe an optimized gene transfer protocol utilizing an ecotropic viral vector to transduce primary murine
Bergren W Crute et al.
Frontiers in immunology, 11, 592329-592329 (2020-11-17)
Among the areas of most impactful recent progress in immunology is the discovery of inhibitory receptors and the subsequent translation of this knowledge to the clinic. Although the original and canonical member of this family is FcγRIIB, more recent studies
Massimo Martinelli et al.
iScience, 26(1), 105860-105860 (2023-01-13)
The RNA-binding protein Pcbp2 is widely expressed in the innate and adaptive immune systems and is essential for mouse development. To determine whether Pcbp2 is required for CD4+ T cell development and function, we derived mice with conditional Pcbp2 deletion in
Anastasiia Kalinina et al.
STAR protocols, 2(1), 100368-100368 (2021-03-23)
Adoptive transfer therapy has great potential to treat diseases such as cancer as well as autoimmune and infectious diseases. Identification of chain-centric T cell receptors (TCRs) with the dominant-active antigen-specific α-chains (TCRα) can significantly improve the efficacy of adoptive cell therapy
Ping-Wei Zhao et al.
Mediators of inflammation, 2015, 762709-762709 (2015-04-22)
This study aimed to assess the differential expression of specific B cell subtypes in patients with chronic viral hepatitis. The frequencies of differential expression of specific B cell subtypes in patients with chronic viral hepatitis and healthy controls were assessed
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.