318337

Acridine Orange hydrochloride hydrate

≥98% (HPLC)

• 동의어(들): 3,6-Bis(dimethylamino)acridine hydrochloride
• 실험식(Hill 표기법): C₁₇H₁₉N₃ · HCl · xH₂O
• CAS Number: 65-61-2
• Molecular Weight: 301.81 (anhydrous basis)
• 색상 지수 번호: 46005
• Beilstein: 3734978
• EC Number: 200-614-0
• MDL number: MFCD00150070
• UNSPSC 코드: 12171500
• PubChem Substance ID: 24859123
• NACRES: NA.47

속성

• Quality Level: 200
• 분석: ≥98% (HPLC)
• 양식: powder
• 기술: microbe id | staining: suitable
• 색상: orange
• mp: 284-287 °C (lit.)
• solubility: H₂O: 0.1%, clear, orange to red
• λmax: 492 nm
• ε (흡광계수): ≥14,000 at 227-233 nm in water at 0.005 g/L; ≥35000 at 265-271 nm in water at 0.005 g/L
• 응용 분야: diagnostic assay manufacturing; hematology; histology
• 저장 온도: room temp
• SMILES string: Cl[H].[H]O[H].CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C
• InChI: 1S/C17H19N3.ClH.H2O/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;/h5-11H,1-4H3;1H;1H2
• InChI key: JUAAUEXLEKAEQC-UHFFFAOYSA-N

설명

애플리케이션

A rigorously purified zinc-free material suitable for use in flow cytometry.

Acridine Orange hydrochloride hydrate dye has been used:

• to stain intracellular acidic vesicles.
• to measure lysosomal membrane permeability (LMP).
• or autophagy detection.

Fluorescent stain for nucleic acids in agarose and polyacrylamide gels. Has been used to study apoptosis in various cell culture and tissue models.

생화학적/생리학적 작용

Acridine Orange is a metachromatic dye which can stain DNA, RNA and acid glycosaminoglycans. At low concentrations, it intercalates into DNA and precipitates RNA. However, at high concentrations it denatures and precipitates both RNA and DNA. Acridine Orange is also used to analyze autophagy. It enters into acidic organelles in a pH-dependent manner. At neutral pH, acridine orange gives a green fluorescence and in acidic conditions, it accumulates in acidic organelles giving a bright red fluorescence.

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안전성 정보

• 픽토그램: GHS08
• 신호어: Warning
• 유해 및 위험 성명서: H341
• 예방조치 성명서: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Muta. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)