A8097

Acridine Orange hydrochloride solution

>95.0% purity (HPLC), solution

• 동의어(들): 3,6-Bis(dimethylamino)acridine hydrochloride
• 실험식(Hill 표기법): C₁₇H₁₉N₃ · HCl
• CAS Number: 65-61-2
• Molecular Weight: 301.81
• Beilstein: 3575587
• MDL number: MFCD00012660
• UNSPSC 코드: 12171500
• PubChem Substance ID: 24891317
• NACRES: NA.47

속성

• product name: Acridine Orange hydrochloride solution, ≥95.0% (HPLC), 10 mg/mL in H₂O
• Quality Level: 200
• 분석: ≥95.0% (HPLC)
• 형태: solution
• 농도: 10 mg/mL in H₂O
• 색상: orange to red
• εmax: 47-54 at 486-492 nm in water
• 응용 분야: diagnostic assay manufacturing; hematology; histology
• 저장 온도: room temp
• SMILES string: Cl[H].CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C
• InChI: 1S/C17H19N3.ClH/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;/h5-11H,1-4H3;1H
• InChI key: VSTHNGLPHBTRMB-UHFFFAOYSA-N

설명

애플리케이션

Acridine Orange hydrochloride solution has been used to study autophagic cell death. It has also been used for the staining of chromosomes.

생화학적/생리학적 작용

Acridine Orange is a metachromatic dye which can stain DNA, RNA and acid glycosaminoglycans. At low concentration it intercalates into DNA and precipitates RNA. However, at high concentration it denatures and precipitates both RNA and DNA. Acridine Orange is also used to analyze autophagy. It goes into acidic organelles in a pH-dependent manner. At neutral pH, acridine orange gives a green fluorescence and in acidic conditions, it accumulates in the acidic organelle giving a bright red fluorescence.

안전성 정보

• 픽토그램: GHS08
• 신호어: Warning
• 유해 및 위험 성명서: H341
• 예방조치 성명서: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Muta. 2
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)