158550

Acridine Orange hemi(zinc chloride) salt

Dye content 85 %

• 동의어(들): 3,6-Bis(dimethylamino)acridine hydrochloride zinc chloride double salt, 3,6-Bis(dimethylamino)acridinium chloride hemi(zinc chloride salt), Basic Orange 14
• Linear Formula: C₁₇H₂₀ClN₃ · HCl · 1/2ZnCl₂
• CAS Number: 10127-02-3
• Molecular Weight: 369.96
• 색상 지수 번호: 46005
• Beilstein: 3734978
• EC Number: 233-353-6
• MDL number: MFCD00081043
• UNSPSC 코드: 12171500
• PubChem Substance ID: 24849765
• NACRES: NA.47

속성

• 양식: powder
• Quality Level: 200
• 구성: Dye content, 85%
• solubility: H₂O: 1 mg/mL, clear, orange to very deep orange
• λmax: 489 nm
• 응용 분야: diagnostic assay manufacturing; hematology; histology
• 저장 온도: room temp
• SMILES string: Cl[H].Cl[H].Cl[Zn]Cl.CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C.CN(C)c4ccc5cc6ccc(cc6nc5c4)N(C)C
• InChI: 1S/2C17H19N3.4ClH.Zn/c2*1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;;;;/h2*5-11H,1-4H3;4*1H;/q;;;;;;+2/p-2
• InChI key: RAHGLSRJKRXOSY-UHFFFAOYSA-L

설명

애플리케이션

Acridine Orange hemi (zinc chloride) salt has been used to stain primary human umbilical vein endothelial cells (HUVEC) and is used in micronucleus tests (MNTs).

Acridine Orange, a cell-permeable metachromatic fluorescent cationic dye that intercalates DNA and RNA, is used in fluorescence and epiflouresence microscopy. Acridine Orange dye has been used to analyze mitochondria and lysosomal content by flow cytometry, characterize multidrug resistance, and measure changes in mitochondrial mass during apoptosis in rat thymocytes.

생화학적/생리학적 작용

Acridine orange (AO) is a fluorescent dye with two different staining characteristics. In fixed tissues, it acts as a metachromatic dye and differentially stains DNA (green) and RNA (orange). But, in living cells it acts as a pH indicator and produce brilliant orange-red in acidic condition. AO has been used to identify apoptotic cells in drosophila embryos. Acridine Orange staining method has been used to deliver more consistent data in the micronucleus test.

안전성 정보

• 픽토그램: GHS08
• 신호어: Warning
• 유해 및 위험 성명서: H341
• 예방조치 성명서: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Muta. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)