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Merck
모든 사진(1)

문서

T9284

Sigma-Aldrich

Triton X-100

BioXtra

동의어(들):

t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether

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About This Item

Linear Formula:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS Number:
Beilstein:
2315025
MDL number:
UNSPSC 코드:
12161900
PubChem Substance ID:
NACRES:
NA.21

생물학적 소스

synthetic

Quality Level

설명

non-ionic

제품 라인

BioXtra

형태

liquid

분자량

micellar avg mol wt 80,000
average mol wt 625

응집 번호

100-155

기술

permeability assay: suitable

불순물

≤0.05% Phosphorus (P)

무기 잔류물

≤0.4%

CMC

0.2-0.9 mM (20-25°C)

전이 온도

cloud point 65 °C
pour point ~7 °C

solubility

water: soluble

density

1.065 g/cm3 at 20 °C (68 °F)

음이온 미량물

chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%

양이온 미량물

Al: ≤0.0005%
Ca: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.0005%
NH4+: ≤0.05%
Na: ≤0.1%
Pb: ≤0.001%
Zn: ≤0.0005%

HLB

13.5

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

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일반 설명

Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

애플리케이션

Triton X-100 has been used:
  • Along with Span® 80 and Tween® 80 in mineral oil for the preparation of oil-phase of microemulsions for PCR technique
  • As a component of NX buffer which is used to pool and break down the microemulsions after PCR cycling
  • In the blocking solution during immunocytochemistry procedure
  • During deglycosylation procedure
  • For membrane solubilization
  • For cell permeabilization
  • In decellularization of porcine heart valve tissue

생화학적/생리학적 작용

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

특징 및 장점

  • BioXtra Grade product for your Cell Biology and Biochemical research
  • Non-ionic nondenaturing detergent
  • Superior wetting agent and emulsifier
  • Highly versatile surfactant

기타 정보

For additional information on our range of Biochemicals, please complete this form.

법적 정보

Span is a registered trademark of Croda International PLC
TWEEN is a registered trademark of Croda International PLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

픽토그램

CorrosionExclamation markEnvironment

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

483.8 °F - closed cup

Flash Point (°C)

251 °C - closed cup

개인 보호 장비

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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프로토콜

Cell staining can be divided into four steps: cell preparation, fixation, application of antibody, and evaluation.

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