A3156

Bovine Serum Albumin

lyophilized powder, γ-irradiated, Globulin Free, BioXtra, suitable for cell culture

• 동의어(들): Albumin bovine serum, BSA, Bovine albumin
• CAS Number: 9048-46-8
• EC Number: 232-936-2
• MDL number: MFCD00130384
• UNSPSC 코드: 12352202
• NACRES: NA.75

속성

• 생물학적 소스: bovine
• Quality Level: 200
• 무균: γ-irradiated
• 제품 라인: BioXtra
• 분석: ≥99% (agarose gel electrophoresis)
• 형태: lyophilized powder
• 분자량: ~66 kDa
• 정제법: cold ethanol and heatshock fractionation
• 기원: USA origin
• 기술: cell culture | mammalian: suitable
• 불순물: ≤10% water (Karl Fischer)
• pH: 4.8-7.5
• solubility: water: soluble (5g/50ml)
• UniProt 수납 번호: P02769
• 배송 상태: ambient
• 저장 온도: 2-8°C
• 유전자 정보: bovine ... ALB(280717)

설명

일반 설명

BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group.

Bovine serum albumin (BSA) is a 66 kDa protein consisting of three domains with two subdomains under each. It is a α-helical, globular and non-glycosylated protein. Albumin is the most abundant plasma protein in humans.

애플리케이션

Bovine serum albumin has been used:

• to prevent sticking of the chromosome to the glass micropipette for elasticity measurements using aspiration
• to block lung homogenates in the plate for mucin protein enzyme-linked immunosorbent assay
• in alginate solution as a protein model for drug release kinetics investigation

생화학적/생리학적 작용

Bovine Serum Albumin (BSA) is a transporter for drugs, hormones and fatty acids. Albumin turnover is seen in infants with iron deficiency anemia. BSA acts as a vital constituent in the cell culture media and favors human embryonic stem cells (hESC) differentiation.

Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

제조 메모

Purified by a combination of heat shock and ethanol fractionation

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)