A4919

Bovine Serum Albumin

heat shock fraction, protease free, low endotoxin, suitable for cell culture, pH 7, ≥98%

• 동의어(들): Albumin bovine serum, BSA, Bovine albumin
• CAS Number: 9048-46-8
• EC Number: 232-936-2
• MDL number: MFCD00130384
• UNSPSC 코드: 12352202
• NACRES: NA.27

속성

• 생물학적 소스: bovine
• Quality Level: 200
• 제품 라인: BioReagent
• 분석: ≥98%
• 형태: lyophilized powder
• 분자량: ~66 kDa
• 정제법: heat shock fractionation
• 포장: poly bottle of
• 기원: USA origin
• 기술: cell culture | mammalian: suitable
• 불순물: protease, none detected; ≤1.0 EU/mg endotoxin
• 손실: ≤5%
• pH: 7
• solubility: water: soluble (40 mg/ml)
• UniProt 수납 번호: P02769
• 외래 활성: BT virus, none detected; Protease by FITC, none detected; VSV virus, none detected
• 저장 온도: 2-8°C
• 유전자 정보: bovine ... ALB(280717)

설명

일반 설명

Bovine serum albumin (BSA), a 66 kDa protein, belongs to the family of serum albumins. It is an α-helical, non-glycosylated and globular protein, that has 17-disulfide bonds.

애플리케이션

Select the right albumin for your application with the Albumin Selection Guide.

Bovine Serum Albumin has been used:

• as a component of cell culture media in cochlear explant culture
• organotypic cultures and embryo culture
• as a component of phosphate-buffered saline (PBS) in immunostaining of human induced pluripotent stem cell (hiPSC)

생화학적/생리학적 작용

Bovine Serum Albumin (BSA) is the most vital constituent of the cell culture media, which aids embryonic stem cells (hESC) differentiation. It acts as a blocking agent in enzyme linked immunosorbent assay (ELISA) to inhibit non-specific binding of antigens and antibodies to the microtiter plates.

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

특징 및 장점

• Tested for use in cell culture
• Protease-free
• Low endotoxin

제조 메모

Prepared using heat shock fractionation

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)