추천 제품
생물학적 소스
bovine
Quality Level
Grade
for molecular biology
제품 라인
BioReagent
양식
solution
정제법
heat shock fractionation
포함
does not contain stabilizer or preservative
기원
USA origin
농도
20 mg/mL in H2O
기술
FISH: suitable
불순물
HIV I and HIVII, HCV and HBsAg, tested negative
UniProt 수납 번호
외래 활성
DNase free
DNase, RNase, NICKase and protease, none detected
NICKase free
Protease free
RNase free
저장 온도
−20°C
유전자 정보
bovine ... ALB(280717)
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일반 설명
Serum albumin is the most extensive plasma protein in the circulatory system. It is produced within the liver and exported as a non-glycosylated protein to the blood plasma. The molecular weight of native serum albumin is around 66 kDa. Native serum albumin is a negatively charged protein.
Acetylation of bovine serum albumin particularly modifies lysine (Lys) residues with elevated frequency, with lesser degrees of modification of serine (Ser) and threonine (Thr) residues. MALDI-TOF mass spectrometry analysis of acetylated BSA has indicated an increased MW of about 3000, compared to native BSA (Ostatná, V. et al., J. Electroanalytical Chem., 821, 97-103 (2018)).. This acetylation process also derivatizes similar amino acid residues in any trace contaminating nucleases in the BSA, to inactivate that nuclease activity. This makes acetylated BSA useful in molecular biology applications where trace nuclease activity must be minimized.
Acetylation of bovine serum albumin particularly modifies lysine (Lys) residues with elevated frequency, with lesser degrees of modification of serine (Ser) and threonine (Thr) residues. MALDI-TOF mass spectrometry analysis of acetylated BSA has indicated an increased MW of about 3000, compared to native BSA (Ostatná, V. et al., J. Electroanalytical Chem., 821, 97-103 (2018)).. This acetylation process also derivatizes similar amino acid residues in any trace contaminating nucleases in the BSA, to inactivate that nuclease activity. This makes acetylated BSA useful in molecular biology applications where trace nuclease activity must be minimized.
애플리케이션
Acetylated BSA has been used as a component of the reaction mixture for adapter ligation. In addition, this B8894 acetylated BSA product has been used in other applications, such as:
- In cDNA library preparation (Hafner, M. et al., RNA, 17(9), 1697-1712 (2011))
- As a blocking agent with streptavidin beads used for capture of biotinylated RNA (Winz, M.-L. et al., Nat. Protocol., 12(1), 122-149 (2017))
Acetylated to inactivate nucleases commonly found in BSA. Acetylated BSA should not be used as a protein standard since acetylation of tyrosine residues prevents color development in the Lowry assay and other similar protein determinations.
생화학적/생리학적 작용
Native serum albumin plays a major role in maintaining the pH and osmotic pressure of the blood. It is also involved in transporting a diverse range of endogenous and exogenous metabolites, such as amino acids, fatty acids, drugs, steroids and metal ions. Native serum albumin can have a role in modulating the levels of Ca2+ and Mg2+ metals in blood.
특징 및 장점
This acetylated BSA product has been tested for the following properties:
- DNase-free / Exonuclease-free
- Nickase-free / Endonuclease-free
- Protease-free
- RNase-free
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
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시험 성적서(COA)
Lot/Batch Number
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