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Merck
모든 사진(8)

문서

G0876

Sigma-Aldrich

β-Glucuronidase from Helix pomatia

Type H-2, aqueous solution, ≥85,000 units/mL

동의어(들):

β-D-Glucuronide glucuronosohydrolase

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About This Item

CAS Number:
효소 위원회 번호:
MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.54

유형

Type H-2

형태

aqueous solution

특이 활성도

≥85,000 units/mL

secondary activity

≤7,500 units/mL sulfatase

배송 상태

wet ice

저장 온도

2-8°C

유사한 제품을 찾으십니까? 방문 제품 비교 안내

애플리케이션

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

생화학적/생리학적 작용

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

품질

Many β-glucuronidases derived from mollusks also contain sulfatase activity.

단위 정의

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

물리적 형태

Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.

픽토그램

Health hazard

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


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문서

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

프로토콜

Enzymatic assay protocol for β-Glucuronidase from Helix Pomatia and Bovine Liver aids laboratory personnel in following procedures.

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