G1544

Anti-GFP, N-terminal antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• 동의어(들): Anti-Green Fluorescent Protein
• MDL number: MFCD05665217
• UNSPSC 코드: 12352203
• NACRES: NA.56

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 양식: buffered aqueous solution
• 농도: ~1.0 mg/mL
• 기술: immunoprecipitation (IP): 1.0-2.5 μg using GFP fusion protein from transfected mammalian cell lysates; western blot: 0.25-0.5 μg/mL using 3-6 ng of purified GFP fusion proteins expressed in mammalian cell extracts
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified

설명

일반 설명

Green fluorescent protein (GFP) chromophore contains hexapeptide fragment with Phe64-Ser-Tyr-Gly-Val-Gln69 aminoacid sequence. GFP structure contains a 11-stranded β-barrel and a central helix and corresponds to a molecular weight of 26 kDa.

특이성

Specific for GFP fusion proteins.

면역원

synthetic peptide corresponding amino acids 3-17 of GFP.

애플리케이션

Anti-GFP, N-terminal antibody produced in rabbit has been used:

• in immunoblotting
• in the histochemistry and immunohistochemistry
• in the immunoprecipitation

생화학적/생리학적 작용

Green fluorescent protein (GFP) has photochemical and diagnostic applications. It is also used widely in protein dynamics studies. The polyclonal anti-GFP, N-terminal rabbit antibody reacts specifically with GFP fusion proteins.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)