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Merck
모든 사진(1)

주요 문서

M7403

Sigma-Aldrich

MCDB 153 Medium

With trace elements, L-glutamine and 28mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

동의어(들):

Molecular, Cellular, and Development Biology 153 medium

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About This Item

UNSPSC 코드:
12352207
NACRES:
NA.75

Quality Level

양식

powder

기술

cell culture | mammalian: suitable

성분

NaHCO3: no
L-glutamine: 0.8772 g/L
glucose: 1.081 g/L (Dextro)
sodium pyruvate: 0.055 g/L
phenol red: 0.001242 g/L
HEPES: 6.6 g/L (25mM)

배송 상태

ambient

저장 온도

2-8°C

일반 설명

MCDB 153 media are modifications of Ham′s nutrient mixture F-12, designed for the growth of human keratinocytes, clonal growth of chicken embryo fibroblasts, and Chinese hamster ovary (CHO) cells using low levels of fetal bovine serum protein (FBSP), extensive trace elements or no serum protein.

애플리케이션

MCDB 153 Medium has been used as a component of melanoma isolation media (MIM) to culture human non-metastatic and metastatic melanoma cells.

수량

Formulated to contain 17.6 grams of powder per liter of medium.

재구성

Supplement with 1.18 g/L sodium bicarbonate.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리 방문

Hans Peter Weitzenböck et al.
Cancer medicine, 11(4), 956-967 (2021-12-25)
Malignant melanoma is the deadliest form of skin cancer and NRF2 has been proposed as a main regulator of tumor cell malignancy. Still the mechanisms how NRF2 is contributing to melanoma progression are incompletely understood. Here we analyzed the effects
Andrew S McNeal et al.
eLife, 10 (2021-11-24)
Benign melanocytic nevi frequently emerge when an acquired BRAFV600E mutation triggers unchecked proliferation and subsequent arrest in melanocytes. Recent observations have challenged the role of oncogene-induced senescence in melanocytic nevus formation, necessitating investigations into alternative mechanisms for the establishment and
Matthew L Fisher et al.
Molecular carcinogenesis, 55(12), 2024-2036 (2015-12-24)
Melanoma is a metastatic cancer associated with poor survival. Here, we study a subpopulation of melanoma cancer cells displaying melanoma cancer stem cell (MCS cells) properties including elevated expression of stem cell markers, increased ability to survive as spheroids, and
Dominik Brokatzky et al.
The EMBO journal, 38(11) (2019-04-14)
Apoptosis is a frequent form of programmed cell death, but the apoptotic signaling pathway can also be engaged at a low level, in the absence of cell death. We here report that such sub-lethal engagement of mitochondrial apoptosis signaling causes
Yochai Wolf et al.
Cell, 179(1), 219-235 (2019-09-17)
Although clonal neo-antigen burden is associated with improved response to immune therapy, the functional basis for this remains unclear. Here we study this question in a novel controlled mouse melanoma model that enables us to explore the effects of intra-tumor

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