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Merck
모든 사진(2)

문서

MAK145

Sigma-Aldrich

Fluorometric Intracellular Ros Kit

sufficient for 200 fluorometric tests (red)

동의어(들):

Intracellular ROS Fluorescence Assay

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About This Item

UNSPSC 코드:
12161503
NACRES:
NA.25

사용

sufficient for 200 fluorometric tests (red)

검출 방법

fluorometric

관련 질환(들)

neurological disorders; cancer

저장 온도

−20°C

일반 설명

Reactive oxygen species (ROS) are generated as a result of the reduction of oxygen during aerobic respiration and by various enzymatic systems within the cell. At physiological levels, ROS contribute to cell signaling and host defense. Increased ROS generation, above the detoxification capacity of the biological system, results in oxidative stress and cellular damage. The main damage to cells results from the ROS-induced alteration of macromolecules such as polyunsaturated fatty acids in membrane lipids, essential proteins, and DNA. ROS has been implicated in disease states, such as Alzheimer′s disease, Parkinson′s disease, cancer, and aging.

애플리케이션

Fluorometric intracellular reactive oxygen species (ROS) Kit has been used for detection of intracellular ROS in live cells.

적합성

This kit is suitable for the detection of intracellular ROS by fluorescence microplate reader or a fluorescent microscope.

원리

The kit provides a sensitive, one-step fluorometric assay to detect intracellular ROS (especially superoxide and hydroxyl radicals) in live cells within 1 hour incubation. ROS react with a cell-permeable sensor, resulting in a fluorometric product (λex = 520/λem = 605 nm) proportional to the amount of ROS present The assay can be performed in either a 96 or 384 multiwell plate format using a fluorescence microplate reader.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants.
Das K and Roychoudhury A
Frontiers in Endocrinology, 2, 53-53 (2014)
Minghui Liu et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 33(4), 5535-5547 (2019-01-17)
Tumor suppressor phosphatase and tensin homolog deleted on chromosome 10 (PTEN) plays a critical role in regulating cell survival, cell growth, and proliferation by antagonizing the PI3K-AKT-mTOR pathway. The regulatory mechanism of PTEN protein is still not completely understood. Here
Comparative in vitro toxicity of a graphene oxide-silver nanocomposite and the pristine counterparts toward macrophages.
Luna L A V, et al.
Journal of Nanobiotechnology, 14(1), 12-12 (2016)
Yara E Elakkad et al.
Breast cancer (Dove Medical Press), 13, 675-689 (2021-12-23)
Female breast cancer is the most prevalent cancer worldwide. Emerging evidence shows that simvastatin (SIM) has promising anticancer activities. However, the underlying mechanisms are not fully elucidated. Increasing reports imply statins can modulate ferroptosis through disrupting reactive oxygen species (ROS)
Jianyong Yu et al.
Experimental and therapeutic medicine, 22(3), 946-946 (2021-07-27)
Salinomycin is a polyether antiprotozoal antibiotic that is widely used as an animal food additive. Some antifungal, antiparasitic, antiviral and anti-inflammatory activities have been reported for salinomycin. Recently, the anti-cancer effect of salinomycin has been demonstrated in breast cancer; however

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