R0884

T7 RNA Polymerase

recombinant, expressed in E. coli, buffered aqueous solution

• 동의어(들): RNA Polymerase T7, RNA Polymerase, T7 from E. coli HMS 174/pAR1219
• CAS Number: 9014-24-8
• 효소 위원회 번호: 2.7.7.6 (BRENDA, IUBMB)
• MDL number: MFCD00132197
• UNSPSC 코드: 12352204

속성

• 재조합: expressed in E. coli
• Grade: for molecular biology
• 양식: buffered aqueous solution
• 분자량: 98.8 kDa
• 농도: 10,000-50,000 U/mL
• UniProt 수납 번호: P00573
• 외래 활성: DNase and RNase, none detected
• 저장 온도: −20°C
• 유전자 정보: bacteriophage T7 ... T7p07(1261050)

설명

일반 설명

T7 RNA polymerase is highly specific for the bacteriophage T7 promoter and terminator sequences. It is extensively used to prepare RNA transcripts for stuctural and metabolic studies. The RNA transcripts can be converted to probes for sensitive hybridization detection studies. T7 polymerase and dideoxynucleotides can be used to directly sequence DNA.

성분

T7 RNA Polymerase is supplied as a solution of 100 mM NaCl, 50 mM Tris-HCl (pH 7.9), 0.1 mM EDTA, 0.1% Triton X-100, 1 mM DTT, and 50% (v/v) glycerol.

단위 정의

One unit will catalyze the incorporation of 1 nmol of rNTP into acid-precipitable material in 60 min at 37°C.

분석 메모

Activity assay: 40 mM Tris-HCl, pH 7.9, 6 mM MgCl₂, 4 mM spermidine, 10 mM DTT, 0.5 μM each rNTP + 10 μCi α-³²P-UTP, 3-10 units of enzyme, and 1 μg of a 350 bp template are incubated for 10 min at 37°C in a total volume of 100 μl. Typical results are ≥50% incorporation of labeled nucleotide into ≥90% full-length transcript.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable