S7320

Anti-Spred-2 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• 동의어(들): Anti-Sprouty Protein with EVH-1 Domain 2
• MDL number: MFCD07784893
• UNSPSC 코드: 12352203
• NACRES: NA.44

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 형태: buffered aqueous solution
• 분자량: ~48 kDa
• 종 반응성: human, mouse, rat
• 농도: ~1 mg/mL
• 기술: indirect immunofluorescence: 5-10 μg/mL using PC-12 cell lines; microarray: suitable; western blot: 1-2 μg/mL using cell extracts of HepG2; western blot: 2-4 μg/mL using mouse liver extracts
• UniProt 수납 번호: Q7Z698
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... SPRED2(200734) ; mouse ... Spred2(114716) ; rat ... Spred2(305539)

설명

일반 설명

Anti-Spred-2 is developed in rabbit using as immunogen a synthetic peptide corresponding to amino acids of mouse Spred-2, conjugated to KLH via an N terminal added cysteine residue. Spred-2, also known as sprouty protein with EVH-1 domain 2 related sequence, was isolated by similarity to Spred-1, which in turn was isolated from an osteoclast complementary DNA library by a two-hybrid system using c-Kit and c-Fms tyrosine kinase domains as bait. These proteins contain a carboxy-terminal cysteine-rich domain related to sprouty (the SPR domain), and an amino-terminal Ena/vasodilator stimulated phosphoprotein (VASP) homology (EVH-1) domain. This gene is mapped to human chromosome 2p14.

Spred proteins mainly function as inhibitors of the ERK and MAPK signaling cascades. Phosphorylation and Cbl-dependent ubiquitination regulate Spred-2 expression levels. Spred2 negatively modulates the hypothalamic-pituitary-adrenal axis . Anti-Spred-2 antibody is specific for human, rat, and mouse Spred-2 (approx. 48 kDa). Staining of the Spred-2 band in immunoblotting is specifically inhibited by the immunizing peptide.

면역원

synthetic peptide corresponding to amino acids 280-297 of mouse Spred 2. The sequence is conserved in rat and differs from human by three amino acids.

애플리케이션

Anti-Spred-2 antibody produced in rabbit has been used in western blotting and indirect immunofluorescence.

생화학적/생리학적 작용

Spred inhibits growth factor mediated activation of MAP kinase. Spred inhibits the activation of MAP kinase probably by mechanisms such as suppression of phosphorylation and activation of Raf. Spred-2 functions as a negative regulator of AGM (aorta-gonad-mesonephros) hematopoiesis by inhibiting hematopoietic cytokine signaling. Spred proteins are also regulators of Rho-mediated cell motility.

물리적 형태

Solution in 0.01 M phoshate buffered saline, pH 7.4, 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)