SRE0019

Apyrase from potatoes

Suitable for manufacturing of diagnostic kits and reagents, ATPase ≥200 units/mg protein

• 동의어(들): Apyrase from potatoes, Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase
• CAS Number: 9000-95-7
• 효소 위원회 번호: 3.6.1.5 (BRENDA, IUBMB)
• EC Number: 232-569-8
• MDL number: MFCD00130542
• UNSPSC 코드: 12352204
• NACRES: NA.32

속성

• Quality Level: 400
• 유형: Grade VII
• 형태: lyophilized powder
• ATPase activity: ≥200 units/mg protein
• secondary activity: ≥50 % of base activity ADPase
• 구성: Protein, 25-60%
• solubility: H₂O: soluble 1.0 mg/mL
• 응용 분야: diagnostic assay manufacturing
• 외래 활성: Acid Phosphatase ≤2% of base activity
• 저장 온도: −20°C

설명

애플리케이션

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation.

At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).
Reaction: ATP → ADP+P_i → AMP+2P_i.

생화학적/생리학적 작용

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.

단위 정의

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

물리적 형태

Partially purified, lyophilized powder containing potassium succinate buffer salts.

제조 메모

Sold on the basis of ATPase units.

기타 정보

This grade has a low ATPase/ADPase ratio.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable