SRP0288

ATP Citrate Lyase Active human

recombinant, expressed in baculovirus, ≥90% (SDS-PAGE)

• 동의어(들): ACLY, ATP-citrate synthase, ATPCL, CLATP
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 재조합: expressed in baculovirus
• 분석: ≥90% (SDS-PAGE)
• 양식: aqueous solution
• 특이 활성도: ≥1.2 pmol/min-μg
• 분자량: 147 kDa
• NCBI 수납 번호: NM_001096
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: mouse ... ACLY(47)

설명

일반 설명

Human ATP citrate lyase (ACLY) (GenBank Accession No. NM_001096), full length, with N-terminal GST tag, MW = 147 kDa, expressed in baculovirus expression system.

애플리케이션

Active human ATP citrate lyase is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Active human ATP citrate lyase has been used in a study to ascertain the nature of the catalytic phosphorylation that initiates the ACL reaction, and to identity the active site residues involved. Active human ATP citrate lyase has also been used in a study to analyze tumor metabolism to reveal mitochondrial glucose oxidation in genetically diverse human glioblastomas.

생화학적/생리학적 작용

ATP citrate lyase is an enzyme involved in fatty acid synthesis that generates cytosolic acetyl-CoA and oxaloacetate from citrate and CoA. ATP citrate lyase is often upregulated in cancer.

단위 정의

One unit is defined as the amount of enzyme required to convert 1 pmol of ADP to ATP/min at 37°C.

물리적 형태

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol.

제조 메모

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable