추천 제품
유형
Type X
형태
lyophilized powder
특이 활성도
≥3,500 units/mg protein
분자량
calculated mol wt 53.2 kDa
구성
Protein, 60-90% biuret
외래 활성
pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%
저장 온도
−20°C
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일반 설명
Triosephosphate isomerase (TPI) gene is located on human chromosome 12p13. It is expressed in all tissues. This homodimer of two 27 kDa subunits is highly stable and is made up of 248 amino acids. TPI is catalytically active in its dimeric state.
애플리케이션
Triosephosphate Isomerase from rabbit muscle has been complexed with p-hydroxymercurybenzoate (PHMB) to denature the protein for hydrophobic interaction chromatography coupled online with chemical vapour atomic fluorescence spectrometry (HIC−CVGAFS).
Triosephosphate isomerase has been used in a study to assess changes in cardiac energy metabolic pathways in overweight rats fed a high-fat diet. Triosephosphate isomerase has also been used in a study to investigate proteomic identification of carbonylated proteins in F344 rat hippocampus after 1-bromopropane exposure.
생화학적/생리학적 작용
Triosephosphate isomerase (TPI) is responsible for the catalysis of the triose phosphate isomers, dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. TPI deficiency is associated with glycolytic enzymopathy. Lack of TPI is also associated with chronic hemolytic anemia, enhanced exposure to bacterial infections, cardiomyopathy and neuromuscular disease.
포장
Package size based on protein content
단위 정의
One unit will convert 1.0 μmole D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.
물리적 형태
Sulfate-free, contains EDTA and borate buffer salts
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Red blood cell enzymopathies
Hematology, 616-625 (2018)
Changes in cardiac energy metabolic pathways in overweighed rats fed a high-fat diet
Modrego, J., et al.
European Journal of Nutrition, DOI: 10-DOI: 10 (2012)
Zhenlie Huang et al.
Toxicology and applied pharmacology, 263(1), 44-52 (2012-06-12)
1-Bromopropane (1-BP) is neurotoxic in both experimental animals and humans. Previous proteomic analysis of rat hippocampus implicated alteration of protein expression in oxidative stress, suggesting that oxidative stress plays a role in 1-BP-induced neurotoxicity. To understand this role at the
Triosephosphate isomerase deficiency: new insights into an enigmatic disease
Orosz F, et al.
Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1792(12), 1168-1174 (2009)
Hydrophobic interaction chromatography coupled with atomic fluorescence spectrometric detection: Effect of the denaturation on the determination of thiolic proteins
Bramanti E, et al.
Talanta, 63(2), 383-389 (2004)
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