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Merck
모든 사진(1)

Key Documents

T7777

Sigma-Aldrich

Tris-Glycine-SDS Buffer 10× Concentrate

동의어(들):

TRIS glycine SDS buffer solution

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About This Item

UNSPSC 코드:
41105319
eCl@ss:
32129211
NACRES:
NA.25

무균

0.2 μm filtered

Quality Level

형태

liquid

응용 분야

diagnostic assay manufacturing

저장 온도

2-8°C

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일반 설명

Tris-glycine-SDS (TGS) running buffer is the most commonly used buffer for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. TGS is usually used for both anode and cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units.

애플리케이션

Tris-glycine-SDS buffer 10× concentrate has been used as a running buffer in sodium dodecyl sulfate-polyacryamide gel electrophoresis (SDS-PAGE). It is also used to block membranes with 5% non-fat dry milk for western blotting. TGS is usually used for both the anode buffer and the cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units.

재구성

Dilution of the 10× TGS buffer produces a 1× running buffer containing 25 mM Tris, 192 mM glycine and 0.1% SDS, pH approx. 8.6.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


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