T4904

Tris-Glycine Buffer 10× Concentrate

• 동의어(들): running buffer
• EC Number: 232-650-8
• UNSPSC 코드: 41105323
• NACRES: NA.25

속성

• 무균: 0.2 μm filtered
• Quality Level: 200
• 외래 활성: Protease, none detected
• 저장 온도: 2-8°C

설명

애플리케이션

Tris-Glycine buffer 10× concentrate has been used as a transfer buffer for Western blotting.

생화학적/생리학적 작용

For Western blotting and gel electrophoresis. Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to nitrocellulose.The 10× Tris-glycine buffer is diluted to 1× with methanol and water to make a solution containing 25 mM Tris, 192 mM glycine, and 20% methanol. A sufficient amount of transfer buffer should be made to cover the electrode wires in the wet blot transfer unit and to soak the gel, membrane and blotting paper.

기타 정보

0.25 M Tris, 1.92 M glycine, pH approx. 8.3
Contains no methanol.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type ABEK (EN14387) respirator filter