G8898

Glycine

≥99%, suitable for western blotting

• 동의어(들): Aminoacetic acid, Aminoethanoic acid, Glycocoll
• Linear Formula: NH₂CH₂COOH
• CAS Number: 56-40-6
• Molecular Weight: 75.07
• Beilstein: 635782
• EC Number: 200-272-2
• MDL number: MFCD00008131
• UNSPSC 코드: 12352209
• eCl@ss: 32160406
• PubChem Substance ID: 24895355
• NACRES: NA.28

속성

• 제품명: Glycine, suitable for electrophoresis, ≥99%
• Quality Level: 200
• 분석: ≥99%
• 양식: powder
• 기술: electrophoresis: suitable; western blot: suitable
• 불순물: ≤0.01% Insoluble matter
• 색상: white to off-white
• pKa(25 °C): (1) 2.35, (2) 9.60; 2.35
• mp: 240 °C (dec.) (lit.)
• solubility: H₂O: 200 mg/mL, clear, colorless to faintly yellow
• 음이온 미량물: chloride (Cl^-): ≤70 ppm
• 양이온 미량물: heavy metals (as Pb): ≤20 ppm
• 흡수: ≤0.15 at 280 at 1 M
• 작용기: amine; carboxylic acid
• 저장 온도: room temp
• SMILES string: NCC(O)=O
• InChI: 1S/C2H5NO2/c3-1-2(4)5/h1,3H2,(H,4,5)
• InChI key: DHMQDGOQFOQNFH-UHFFFAOYSA-N
• 유전자 정보: rat ... Grin2a(24409)

설명

일반 설명

Glycine is a molecule with pI of 6.7, which is similar to the pH of stacking gel. It has advantage of low mobility, hydrophobicity and it does not associate with proteins. Glycine is a component of Tris-glycine and Tris-glycine-SDS (sodium dodecyl sulfate) running buffers for polyacrylamide gel electrophoresis. Glycine is also a component of Towbin′s transfer buffer for Western blots.

Glycine, an achiral proteinogenic amino acid, serves diverse roles in biochemistry and physiology. As a non-essential, non-polar glucogenic amino acid, it acts as an inhibitory neurotransmitter in the central nervous system and co-agonist at NMDA receptors with glutamate. Serving as a precursor, glycine is a crucial building block for macromolecules, playing a vital role in cellular processes.

In addition to its biological functions, glycine exhibits versatility in research applications. Its role as a biochemical reagent makes it valuable in assays and procedures, while its buffering capacity is essential in techniques like SDS-PAGE, Western Blotting, chromatography, and cell culture. Glycine′s zwitterionic nature makes it an effective buffer across various pH values, widely used in immunology research for buffer preparation in techniques such as Western Blotting. Compatible with enzymes, glycine is useful in enzymatic assays like lactate determination. Moreover, glycine contributes to buffer formulations for protein stabilization, pH control, and enzymatic reactions.

애플리케이션

• Glycine has been used for the preparation of gelatin composites scaffold.
• It has been used in the elution buffer to extract protein using HiTrap protein G column.
• It has been used in the SDS-sample, running and transfer buffers prepared for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and western blotting.

생화학적/생리학적 작용

Inhibitory neurotransmitter in spinal cord, allosteric regulator of NMDA receptors.

특징 및 장점

• Suitable for Cell Biology and Biochemical studies
• High-quality compound suitable for multiple research applications

분석 메모

Tested for use in electrode buffers for PAGE and in transfer buffers for Western blots.

기타 정보

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안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)