ZRB1621

Anti-phospho-ULK1-Ser757 Antibody, clone 5L18 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 동의어(들): ATG1, Autophagy-related protein 1 homolog, EC:2.7.11.1, Serine/threonine-protein kinase ULK1, Unc-51-like kinase 1, hATG1
• UNSPSC 코드: 12352203
• NACRES: NA.43

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 재조합: expressed in HEK 293 cells
• 결합: unconjugated
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 5L18, monoclonal; recombinant monoclonal
• 제품 라인: ZooMAb^® learn more
• 형태: lyophilized
• 분자량: calculated mol wt 112.63 kDa; observed mol wt ~112 kDa
• 종 반응성: human
• 종 반응성(상동성에 의해 예측): sheep, bovine
• 포장: antibody small pack of 25 μL
• 환경친화적 대안 제품 특성: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 향상된 검증: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 기술: affinity binding assay: suitable; inhibition assay: suitable (peptide); western blot: suitable
• 동형: IgG
• UniProt 수납 번호: O75385
• 환경친화적 대안 카테고리: Aligned,
• 배송 상태: ambient
• 저장 온도: 2-8°C
• 타겟 번역 후 변형: phosphorylation (pSer75)
• 유전자 정보: human ... ULK1(8408)

설명

일반 설명

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

특이성

Clone 5L18 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects ULK1 phosphorylated on serine 757.

면역원

KLH-conjugated linear peptide corresponding to 13 amino acids surrounding phosphoserine 757 from the C-terminal half of human ULK1.

애플리케이션

Anti-phospho-ULK1-Ser757, clone 5L18 ZooMAb, Cat. No. ZRB1621, is a recombinant Rabbit monoclonal antibody that targets ULK1 phosphorylated on serine 757 and is tested in Affinity Binding Assay and Peptide Inhibition Assay, and Western Blotting.

Peptide Inhibition Assay: Target band detection in lysate from serum starved A431 cells was prevented by preblocking of a representative lot (1:1,000 dilution) with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.

Affinity Binding Assay: A representative lot of this antibody bound phosphorylated ULK1 (pSer757) peptide with a KD of 8.2 x 10-6 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

표적 설명

Serine/threonine-protein kinase ULK1 (UniProt: O75385; also known as EC:2.7.11.1, Autophagy-related protein 1 homolog, ATG1, hATG1, Unc-51-like kinase 1) is encoded by the ULK1 (also known as KIAA0722) gene (Gene ID: 8408) in human. ULK1 is a ubiquitously expressed serine-threonine protein kinase that is involved in autophagy in response to starvation. During starvation it is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome. In cells, ULK1 is constitutively in complex with at least three proteins: ATG13, FIP200 and ATG101. Interaction of ULK1 with ATG13 or FIP200 is reported to increase ULK1 kinase activity and stability. ULK1 undergoes autophosphorylation under nutrient-rich conditions and is dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency, it is phosphorylated by MTOR/mTOR, which disrupts the interaction with AMPK and prevents activation of ULK1. In response to nutrient limitation it is phosphorylated and activated by AMPK that leads to activation of autophagy. It can also act as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulation of AMPK activity. Under nutrient sufficiency, high mTOR activity is reported to prevent ULK1 activation by phosphorylating ULK1 on serine 757 and disrupt its interaction with AMPK. The protein kinase domain of ULK1 is localized to amino acids 16-278. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zachari, M., and Ganley, IG. (2017). Essays Biochem. 61(6); 585-596; Kim, J., et al. (2011). Nat. Cell Biol. 13(2); 132-141).

물리적 형태

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

재구성

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

법적 정보

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1