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PhotoCol-RUT, methacrylated collagen bioink kit, with ruthenium

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3D Bioprinting, Bioink, Collagen


Methacrylated collagen:
Degree of methacrylation ≥ 20%

Product components :
Methacrylated collagen (100 mg)
20 mM acetic acid (50 mL)
Neutralization solution (10 mL)

Ruthenium (100 mg)
Sodium persulfate photoinitiator (500 mg)

Quality Level


sterile; sterile-filtered


≤10 EU/mL Endotoxin

storage temp.



PhotoCol-RUT bioink kit consists of purified methacrylated Type I bovine collagen as the core component with other support reagents. The methacrylated Type I collagen is produced from telo-peptide intact bovine collagen where the collagen has been modified by reacting the free amines, primarily the ε-amines groups of the lysine residues as well as the α-amines groups on the N-termini. Over 20% of the total lysine residues of the collagen molecule have been methacrylated. A bottle of 20 mM acetic acid solution is provided to solubilize the lyophilized methacrylated collagen at concentrations ranging from 3 to 8 mg/ml. The neutralization solution consists of an alkaline 10X phosphate buffered saline (PBS) solution which provides physiological salts and pH in the final mixture. The photoinitiator consists of ruthenium and sodium persulfate to be formulated in 1X cell culture media or PBS, which allows visible light photocrosslinking of the printed structure at 400-450 nm. PhotoCol-RUT provides native-like 3D collagen gels, and the final gel stiffness can be customized by changing collagen concentrations and crosslinking.

Legal Information

PhotoCol is a trademark of Advanced BioMatrix, Inc.



Hazard Statements

Precautionary Statements

Hazard Classifications

Aquatic Chronic 2

Storage Class Code

10 - Combustible liquids



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Apekshya Chhetri et al.
Current protocols in chemical biology, 11(2), e65-e65 (2019-06-06)
With the increase in knowledge on the importance of the tumor microenvironment, cell culture models of cancers can be adapted to better recapitulate physiologically relevant situations. Three main microenvironmental factors influence tumor phenotype: the biochemical components that stimulate cells, the
Andrea Mazzocchi et al.
ACS biomaterials science & engineering, 5(4), 1937-1943 (2019-11-15)
Lung cancer is the leading cause of cancer-related death worldwide yet in vitro disease models have been limited to traditional 2D culture utilizing cancer cell lines. In contrast, recently developed 3D models (organoids) have been adopted by researchers to improve
Andrea Mazzocchi et al.
Biofabrication, 11(1), 015003-015003 (2018-10-03)
Current 3D printing of tissue is restricted by the use of biomaterials that do not recapitulate the native properties of the extracellular matrix (ECM). These restrictions have thus far prevented optimization of composition and structure of the in vivo tissue
Mohammad Izadifar et al.
Tissue engineering. Part C, Methods, 24(2), 74-88 (2017-10-21)
Biofabrication of cell supportive cardiac patches that can be directly implanted on myocardial infarct is a potential solution for myocardial infarction repair. Ideally, cardiac patches should be able to mimic myocardium extracellular matrix for rapid integration with the host tissue
Kathryn E Drzewiecki et al.
Langmuir : the ACS journal of surfaces and colloids, 30(37), 11204-11211 (2014-09-11)
Type-I collagen self-assembles into a fibrillar gel at physiological temperature and pH to provide a cell-adhesive, supportive, structural network. As such, it is an attractive, popular scaffold for in vitro evaluations of cellular behavior and for tissue engineering applications. In

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