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1.04119

Osmium(VIII) oxide

for microscopy

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Synonym(s):
Osmium(VIII) oxide
Empirical Formula (Hill Notation):
O4Os
CAS Number:
Molecular Weight:
254.23
MDL number:

vapor pressure

10 hPa ( 20 °C)

Quality Level

potency

15 mg/kg LD50, oral (Rat)

color

yellow

pH

6 (20 °C, 25 g/L in H2O)

bp

130 °C/1013 hPa

mp

40 °C (decomposition)

solubility

65 g/L

density

4.91 g/cm3 at 20 °C

bulk density

1500 kg/m3

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

15-25°C

InChI

1S/4O.Os

InChI key

VUVGYHUDAICLFK-UHFFFAOYSA-N

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vibrant-m

1.04119

Osmium(VIII) oxide

-
Osmium tetroxide Sealed ampule.

Sigma-Aldrich

O5500

Osmium tetroxide

-
Osmium tetroxide ReagentPlus®, 99.8%

Sigma-Aldrich

201030

Osmium tetroxide

-
Osmium tetroxide ACS reagent, ≥98.0%

Sigma-Aldrich

419494

Osmium tetroxide

Essential Grade
pH

6 (20 °C, 25 g/L in H2O)

pH

-

pH

-

pH

-

mp

40 °C (decomposition)

mp

39.5-41 °C (lit.)

mp

39.5-41 °C (lit.)

mp

39.5-41 °C (lit.)

solubility

65 g/L

solubility

-

solubility

-

solubility

acetone: soluble(lit.), carbon tetrachloride: soluble(lit.), methyl tert-butyl ether: soluble(lit.), toluene: soluble(lit.), water: soluble(lit.)

density

4.91 g/cm3 at 20 °C

density

-

density

-

density

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

-

application(s)

-

General description

Osmium tetroxide for microscopy can be used to visualize unsaturated lipids. A part of the lipids is immediately blackened by the influence of osmic acid solution. The immediate black coloration results from the -C=C- double bond of the unsaturated fatty acid. The fatty acids that are made brownish at first can be blackened by treatment with 60-70% alcohol. For electron microscopy or semithin sectioning the glutardialdehyde-fixed specimens are post-fixed with osmium(VIII) oxide. Lipid inclusions appear black on a yellow to brown background.

Application

for microscopy

Analysis Note

Identity (Raman-spectrum): conforms

Pictograms

CorrosionSkull and crossbones

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 1 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Eye Dam. 1 - Skin Corr. 1B

Storage Class Code

6.1B - Non-combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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