MilliporeSigma
All Photos(3)

Documents

AB356492M

Sigma-Aldrich

Anti-Cas9 (Chicken Polyclonal)

from chicken

Sign Into View Organizational & Contract Pricing

Synonym(s):
SaCas9, CRISPR-associated endonuclease Cas9
eCl@ss:
32160702

biological source

chicken

Quality Level

antibody form

unpurified

antibody product type

primary antibodies

clone

polyclonal

species reactivity

bacteria, Staphylococcus aureus

technique(s)

immunofluorescence: suitable
western blot: suitable

isotype

IgY

UniProt accession no.

target post-translational modification

unmodified

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
AB356480MAB131872ABS2202
Anti-Cas9 (Chicken Polyclonal) from chicken

AB356492M

Anti-Cas9 (Chicken Polyclonal)

Anti-Cas9 (Rabbit Polyclonal) serum, from rabbit

AB356480

Anti-Cas9 (Rabbit Polyclonal)

Anti-Cas9 Antibody, clone 6F7 clone 6F7, from mouse

MAB131872

Anti-Cas9 Antibody, clone 6F7

Anti-Cas9 from rabbit, purified by affinity chromatography

ABS2202

Anti-Cas9

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

antibody form

unpurified

antibody form

serum

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

technique(s)

immunofluorescence: suitable, western blot: suitable

technique(s)

immunofluorescence: suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

clone

polyclonal

clone

polyclonal

clone

6F7, monoclonal

clone

polyclonal

species reactivity

bacteria, Staphylococcus aureus

species reactivity

Staphylococcus aureus, bacteria

species reactivity

Staphylococcus aureus, mouse

species reactivity

Streptococcus pyogenes, bacteria

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

CRISPR-associated endonuclease Cas9 (UniProt: J7RUA5; also known as SaCas9, Cas9) is encoded by the Cas9 gene in Streptococcus aureus. Cas9 belongs to subtype II-A subfamily with one HNH Cas9-type domain. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. Cas9 has a bi-lobed architecture with a recognition lobe (residues 41-425) and a discontinuous nuclease lobe (NUC, residues 1-40 and 453-1053); the crRNA-target DNA lies in a channel between the 2 lobes. The NUC lobe has 2 endonuclease domains. The discontinuous RuvC-like domain in NUC cleaves the target DNA non-complementary to crRNA while the HNH nuclease domain cleaves the target DNA complementary to crRNA. Cas9 serves as a RNA-guided DNA endonuclease that cleaves double-stranded DNA targets with a protospacer adjacent motif (PAM) and complementarity to the guide RNA. The homologous region of the Streptococcus pyogenes is not closely related in amino acid sequence in S. aureus, hence this antibody does not recognize that protein.

Specificity

This chicken polyclonal antibody detects Cas9 from Staphylococcus aureus. It targets an epitope within 251 amino acids from the C-terminal end. This antibody does not react with Cas9 from Streptococcus pyogenes.

Immunogen

Epitope: C-terminus
His-tagged recombinant fragment corresponding to 251 amino acids from the C-terminal end of CRISPR-associated endonuclease Cas9 from Staphylococcus aureus.

Application

Anti-Cas9 (Chicken Polyclonal), Cat. No. AB356492, is a highly specific chicken polyclonal antibody that targets CRISPR-associated endonuclease Cas9 and has been tested in Immunofluorescence and Western Blotting.
Research Category
Epigenetics & Nuclear Function
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Cas9 in HEK293 cells transfceted with GFP-Cas9 from Staphylococcus aureus (Courtesy of Dr. Gerry Shaw).

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Cas9 in HEK293 cells transfceted with GFP-Cas9 from Staphylococcus aureus (Courtesy of Dr. Gerry Shaw).

Quality

Evaluated by Western Blotting in HEK293 cells transfected with GFP-Cas9 from Staphylococcus aureus.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Cas9 in 40 µg of lysate from HEK293 cells transfected with GFP-Cas9 from Staphylococcus aureus.

Target description

~57 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physical form

Chicken polyclonal antibody in PBS with 0.02% sodium azide.
Format: Unpurified
Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service