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IM27L

Sigma-Aldrich

Anti-Cathepsin B (Ab-1) Mouse mAb (CA10)

lyophilized, clone CA10, Calbiochem®

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biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

CA10, monoclonal

form

lyophilized

does not contain

preservative

species reactivity

porcine, human, bovine

should not react with

rat

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

isotype

IgG2a

shipped in

ambient

storage temp.

2-8°C

Gene Information

mouse ... Ctsb(13030)

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clone

CA10, monoclonal

clone

polyclonal

clone

DH01, monoclonal

clone

FE9, monoclonal

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

biological source

mouse

biological source

rabbit

biological source

mouse

biological source

mouse

Gene Information

mouse ... Ctsb(13030)

Gene Information

-

Gene Information

human ... CDH1(999)

Gene Information

human ... APC(324)

shipped in

ambient

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

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General description

Anti-Cathepsin B (Ab-1), mouse monoclonal, clone CA10, recognizes the ~43 kDa latent and ~31 kDa active forms of cathepsin B in HepG2 cells. It is validated for WB, IF, and IP.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/O-NS1 plasmacytoma cells. Recognizes the ~43 kDa latent and the ~31 kDa active forms of cathepsin B.
Recognizes the ~43 kDa latent and the ~31 kDa active forms of cathepsin B in HepG2 cells.

Immunogen

Epitope: within the heavy chain of mature cathepsin B
Human
purified human liver cathepsin B

Application

Immunoblotting (250 ng/ml)

Immunoprecipitation (1 µg/ml)

Immunofluorescence (see comments)

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from 20 mM ammonium bicabonate buffer, 100 µg BSA (100 µg size) or 20 µg BSA (20 µg size).

Reconstitution

Resuspend the lyophilized antibody with 1 ml sterile PBS. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Freezing of aliquots is best for long-term storage of reconstituted product. Avoid freeze/thaw cycles.

Analysis Note

Positive Control
Hep G2 cells

Other Notes

Sloane, B.F. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1:137-152.
Barret, A.J. 1987. Trends Biochem. Sci.12, 193.
Chan, S.J., et al. 1986. Proc. Natl. Acad. Sci. USA83, 7721.
Barrett, A.J. and Kirschke, H. 1981. Meth. Enzymol.80, 535.
This antibody recognizes an epitope on the heavy chain of mature cathepsin B and therefore detects both mature and pro-cathepsin B. Also reported to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Zhiyu Chen et al.
Cancer letters, 348(1-2), 156-166 (2014-03-26)
The tumor necrosis factor related apoptosis-inducing ligand (TRAIL) causes cancer cell death, but many cancers, including pancreatic cancer, are resistant to TRAIL therapy. A combination of TRAIL and the diterpene triepoxide, triptolide, is effective in inducing pancreatic cancer cell death.
Betty Luong et al.
PloS one, 13(9), e0203053-e0203053 (2018-09-12)
The vacuolar-type H+-ATPase (v-ATPase) is the major proton pump that acidifies intracellular compartments of eukaryotic cells. Since the inhibition of v-ATPase resulted in anti-tumor and anti-metastatic effects in different tumor models, this enzyme has emerged as promising strategy against cancer.
Maksim V Baranov et al.
iScience, 11, 160-177 (2019-01-07)
Antigen presentation to T cells in major histocompatibility complex class II (MHC class II) requires the conversion of early endo/phagosomes into lysosomes by a process called maturation. Maturation is driven by the phosphoinositide kinase PIKfyve. Blocking PIKfyve activity by small molecule
Sonia Boulakirba et al.
Scientific reports, 8(1), 256-256 (2018-01-13)
CSF-1 and IL-34 share the CSF-1 receptor and no differences have been reported in the signaling pathways triggered by both ligands in human monocytes. IL-34 promotes the differentiation and survival of monocytes, macrophages and osteoclasts, as CSF-1 does. However, IL-34
Patrick P Rose et al.
Journal of virology, 81(15), 8050-8062 (2007-05-18)
Kaposi's sarcoma-associated herpesvirus (KSHV) is the pathological agent of Kaposi's sarcoma (KS), a tumor characterized by aberrant proliferation of endothelial-cell-derived spindle cells. Since in many cancers tumorigenesis is associated with an increase in the activity of the cathepsin family, we

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