11271164001

Interleukin-2, mouse (mIL-2)

recombinant (E. coli)

• Synonym(s): mIL-2, mouse interleukin-2
• UNSPSC Code: 12352207

Properties

• biological source: mouse
• Quality Level: 100
• recombinant: expressed in E. coli
• assay: >95% (SDS-PAGE)
• form: solution
• potency: <0.5 ng/mL EC₅₀
• mol wt: 17,200 Da
• packaging: pkg of 10,000 U (5 μg, 1 ml)
• manufacturer/tradename: Roche
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): inhibition assay: suitable
• impurities: <0.1 EU/μgtested (LAL test)
• UniProt accession no.: P04351
• storage temp.: −20°C
• Gene Information: mouse ... Il2(16183)

Description

General description

Contents: 10,000 U/ml solution in PBS and 1 mg/ml BSA, filtered through a 0.2 μm pore size membrane

Interleukin-2 (IL-2, also known as T-cell growth factor, TCGF) is a lymphokine produced by lectin- or antigen-activated T-cells. It plays an important immunoregulatory role. This factor was first identified by its ability to promote the long-term in vitro proliferation of activated T-cells. It also promotes the generation and proliferation of cytotoxic T-cells, natural killer (NK) cells, and lymphokine-activated killer (LAK) cells. IL-2 also induces other lymphokines such as interferon-γ and B-cell growth factor (BCGF-1).

Recombinant Interleukin-2, mouse (mIL-2), is produced in E. coli and purified by standard chromatographic methods.

Specificity

Mouse IL-2 is effective on mouse cells, but not on human cells.

Application

Recombinant murine IL-2 is produced in E. coli and exhibits the following:

• Supports the growth of murine CTLL cells (murine T cell line), but not that of human T-cells.
• Strongly inhibits the binding of recombinant human IL-2 to murine responder cells.
• Weakly inhibits the binding to human responder cells.
• Shares identical biological and immunological activities with human IL-2.
• Is a convenient tool for extensive studies of the pharmacological and physiological activities of IL-2 in murine models.
• used for the stimulation of murine T-cells

Quality

Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)

Sequence

Chain Length 149 AA

One polypeptide chain (149 amino acids), identical to natural mouse IL-2, but not glycosylated. Glycosylation is not essential for biological activity.

Unit Definition

EC₅₀ definition: The amount of mIL-2 that is required to support half-maximal stimulation of cell proliferation (XTT cleavage) with CTLL-2 cells (1 unit equals ≤0.5 ng).

Preparation Note

Working solution: Dilute the concentrated IL-2 solution (10,000 U/ml) with PBS or culture medium containing 1 mg/ml BSA [or HSA (human serum alburnin)], or 1 to 10% serum (v/v).
Storage conditions (working solution): -15 to -25 °C
Note: Avoid repeated freezing and thawing.

Other Notes

Specific activity/EC ₅₀: >2 x 10⁶ U/mg, <0.5 ng/ml (hIL-2, NIBSC, 1^st international standard, 86/504), at least the same specific activity (EC₅₀) compared to the indicated standard is guaranteed.
Note: The biological activity of this product may vary in different in vitro applications. Determine the optimal concentration range for specific applications.
EC ₅₀ definition/Unit definition: The amount of mIL-2 that is required to support half-maximal stimulation of cell proliferation (XTT cleavage) with CTLL-2 cells (1 unit equals =0.5 ng).

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: nwg
• flash_point_f: No data available
• flash_point_c: No data available