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Roche

Cell Proliferation ELISA, BrdU (chemiluminescent)

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Synonym(s):
BrdU, cell proliferation

usage

sufficient for ≤1,000 tests

Quality Level

manufacturer/tradename

Roche

technique(s)

ELISA: suitable

detection method

chemiluminescent

shipped in

wet ice

storage temp.

2-8°C

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1164722900111299964001QIA58
vibrant-m

QIA58

BrdU Cell Proliferation Assay

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Calbiochem®

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

-

technique(s)

-

usage

sufficient for ≤1,000 tests

usage

sufficient for ≤1,000 tests

usage

sufficient for ≤100 tests

usage

sufficient for 1000 tests, sufficient for 200 tests

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

wet ice

detection method

chemiluminescent

detection method

colorimetric

detection method

-

detection method

colorimetric

General description

Chemiluminescent immunoassay for the quantification of cell proliferation, based on the measurement of BrdU incorporation during DNA synthesis:  a nonradioactive alternative to the [3H]-thymidine incorporation assay.

Specificity

The antibody conjugate reacts with the thymidine analogue 5-bromo-2′-deoxyuridine (BrdU) and with BrdU incorporated into DNA. For binding to BrdU incorporated into the DNA, the BrdU-labeled DNA has to be denatured. The antibody does not cross-react with any endogenous cellular components such as thymidine, uridine, or DNA.

Application

Cell Proliferation ELISA, BrdU (chemiluminescent) has been used:
  • for bromodeoxyuridine (BrdU) proliferation assay in ReN and primary astrocytes
  • to test the effect on imatinib on the proliferation of neuroblastoma cells using BrdU incorporation assay
  • to check the effect of palbociclib on cell cycle inhibition using BrdU incorporation in pancreatic patient-derived primary cell lines

The Cell Proliferation ELISA, BrdU (chemiluminescent) belongs to the second, improved generation of kits for measuring DNA synthesis. It is a precise, fast, and simple nonradioactive alternative to quantitate cell proliferation based on the measurement of BrdU incorporation during DNA synthesis in replicating (cycling) cells. Thus, the Cell Proliferation ELISA can be used in many different in vitro cell systems. For example:
  • Detection and quantification of cell proliferation induced by growth factors and cytokines
  • Determination of the inhibitory or stimulatory effects of various compounds on cell proliferation in environmental and biomedical research, and in the food, cosmetic, and pharmaceutical industries
  • Measurement of the immunoreactivity of lymphocytes, stimulated by mitogens or antigens
  • Analysis of the chemosensitivity of tumor cells to different cytostatic drugs in medical research

Packaging

1 kit containing 7 components.

Components

  1. BrdU Labeling Reagent, 1,000x conc
  2. FixDenat
  3. Anti-BrdU-POD 4. Antibody Dilution Solution
  4. Washing Buffer PBS, 10x conc.
  5. Substrate Component A
  6. Substrate Component B

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

Product No.
Description

  • BrdU Labeling Reagent

  • FixDenat ready-to-use

  • Anti-BrdU-peroxidase antibody

  • Antibody Dilution Solution ready-to-use

  • ready-to-use 10x concentrated

  • Substrate Component A

  • Substrate Component B

pictograms

FlameExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Skin Sens. 1

Storage Class

3 - Flammable liquids

wgk_germany

WGK 2


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Xiao-Feng Wang et al.
Molecular medicine reports, 12(4), 5917-5923 (2015-08-25)
Epirubicin, an anthracycline derivative, is one of the main line treatments for brain tumors. The aim of the present study was to verify that epirubicin alters the growth and morphological characteristics of U‑87 glioma cells. In the present study, the
Valerio Ciccone et al.
Oncotarget, 9(17), 13353-13365 (2018-03-24)
Nitric oxide (NO) exerts conflicting effect on tumor growth and progression, depending on its concentration. We aimed to characterize the anti-cancer activity of a new NO donor, Ni(SalPipNONO) belonging to the class of metal-nonoates, in epithelial derived tumor cells, finally
Caixia An et al.
International journal of oncology, 54(4), 1195-1208 (2019-04-11)
Emerging studies have reported that coatomer protein complex subunit β2 (COPB2) is overexpressed in several types of malignant tumor; however, to the best of our knowledge, no studies regarding COPB2 in gastric cancer have been published thus far. Therefore, the present study
Tino Schneider et al.
Glycobiology, 25(8), 812-824 (2015-04-17)
The present study demonstrates that fucose-containing sulfated polysaccharides (FCSP) from brown algae interfere with the CXCL12/CXCR4 axis in human Burkitt's lymphoma cells by binding CXCL12 and thereby blocking both CXCL12-induced CXCR4 receptor activation and downstream effects like migration and secretion
Xin Li et al.
European journal of histochemistry : EJH, 63(4) (2019-12-14)
Serine-arginine protein kinase (SRPK) belongs to a class of cell cycle regulating kinases that can phosphorylate proteins containing serine/arginine-Rich (SR) regions. SR proteins are a family of RNA binding phosphoproteins that control both constitutive and alternative pre-mRNA splicing events. However

Articles

Regulation of the cell cycle involves processes crucial to the survival of a cell, including the detection and repair of genetic damage as well as the prevention of uncontrolled cell division associated with cancer. The cell cycle is a four-stage process in which the cell 1) increases in size (G1-stage), 2) copies its DNA (synthesis, S-stage), 3) prepares to divide (G2-stage), and 4) divides (mitosis, M-stage). Due to their anionic nature, nucleoside triphosphates (NTPs), the building blocks of both RNA and DNA, do not permeate cell membranes.

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

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