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RDD038

Sigma-Aldrich

Sodium phosphate dibasic

BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99.0%, free-flowing, Redi-Dri

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Synonym(s):
sec-Sodium phosphate, Disodium hydrogen phosphate, Disodium phosphate, Sodium hydrogenphosphate
Linear Formula:
Na2HPO4
CAS Number:
Molecular Weight:
141.96
MDL number:

product line

BioReagent
Redi-Dri

Quality Level

assay

≥99.0%

form

powder or crystals

quality

free-flowing

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable

pH

8.9-9.2 (25 °C, 50 g/L in water)

pKa (25 °C)

(1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid)

solubility

water: soluble 100 mg/mL

application(s)

diagnostic assay manufacturing

SMILES string

[Na+].[Na+].[H]OP([O-])([O-])=O

InChI

1S/2Na.H3O4P/c;;1-5(2,3)4/h;;(H3,1,2,3,4)/q2*+1;/p-2

InChI key

BNIILDVGGAEEIG-UHFFFAOYSA-L

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RDD038

Sodium phosphate dibasic

Essential+ Grade
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RDD022

Sodium phosphate dibasic

-
vibrant-m

S3264

Sodium phosphate dibasic

-
vibrant-m

71636

Sodium phosphate dibasic

Premium Grade
assay

≥99.0%

assay

≥99.0%

assay

≥98.5% (titration)

assay

≥99.5% (T)

form

powder or crystals

form

powder

form

powder

form

coarse powder, fine crystals

quality

free-flowing

quality

free-flowing

quality

-

quality

-

technique(s)

cell culture | insect: suitable

technique(s)

-

technique(s)

-

technique(s)

-

pH

8.9-9.2 (25 °C, 50 g/L in water)

pH

8.9-9.2 (25 °C, 50 g/L in water)

pH

8.9-9.2 (25 °C, 50 g/L in water)

pH

8.8-9.4 (25 °C, 0.5 M in H2O)

General description

Sodium phosphate dibasic is a component in many biological buffers used in biochemical, biomedical, and molecular biological research. It is also known as disodium hydrogen phosphate (Na2HPO4), it is an inorganic salt that is highly soluble in water. It serves as a versatile buffering agent in research with a pH of 8.9 - 9.2. It is essential for making several phosphate buffers including PBS, utilized for cell culture, immunohistochemistry, Western blot, ELISA, and other applications where a stable pH is critical.

Application

Sodium phosphate dibasic may be used for phosphate buffer and Phosphate Buffered Saline (PBS) that is commonly utilized for cell culture, immunohistochemistry, western blot, and other biochemical applications where a stable pH is crucial. Including protein purification and enzymatic assays.

Features and Benefits

  • Qualified for use in cell culture; suitable for insect cell culture
  • Redi-Dri packaging controls moisture for hygroscopic chemicals that commonly clump
  • Anhydrous powder remains free-flowing and easy to use
  • Safer to handle and weigh out for routine use in lab
  • Eliminates clumps, maintaining product moisture, quality, and attributes

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Articles

Redi-Dri™ prevents hygroscopic powders, such as inorganic salts, from absorbing moisture and forming clumps, leaving the salts free-flowing every time.

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